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Updated: Aug 6, 2025

Determination of the Mating Efficiency of Haploids in Saccharomyces cerevisiae
Published on: December 2, 2022
Evolution and functional diversification of yeast sugar transporters
Lorena Donzella1,2, Maria João Sousa2, John P Morrissey1
1School of Microbiology, Environmental Research Institute, APC Microbiome Ireland, SUSFERM Research Centre, University College Cork, T12 K8AF, Cork, Ireland.
This study analyzes major facilitator superfamily (MFS) sugar transporters in budding yeasts, revealing their diversity and evolutionary patterns. Understanding these transporters is key for yeast evolution insights and biotechnological applications.
Area of Science:
- Microbiology
- Molecular Biology
- Evolutionary Biology
Background:
- Simple sugars are primary carbon sources for yeasts, with uptake dependent on specific transporters.
- Major facilitator superfamily (MFS) proteins are the most common yeast sugar transporters.
- Information on many MFS transporters is limited, especially those absent in model yeast like Saccharomyces cerevisiae.
Purpose of the Study:
- To investigate the full range of MFS proteins in representative budding yeasts (Saccharomycotina).
- To understand the diversity and evolution of sugar transporters in yeasts.
- To correct misassigned protein functions and identify tailored transporters for biotechnology.
Main Methods:
- Comprehensive phylogenetic analysis of 139 putative sugar transporters.
- Data retrieved from 10 complete yeast genomes.
- Comparative genomics and evolutionary analysis.
Main Results:
- Identified extensive diversity within the MFS transporter family across budding yeasts.
- Phylogenetic analysis corrected putative functions of several sugar transporters.
- Gene expansion patterns correlate with the importance of specific sugar transport in different yeast species.
Conclusions:
- This study provides a clearer understanding of yeast sugar transporter evolution and diversity.
- Corrected functional assignments enhance the accuracy of transporter studies.
- The findings offer a valuable resource for selecting or designing specific transporters for biotechnological purposes.
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