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Salinibacterium sedimenticola sp. nov., Isolated from Tidal Flat Sediment
Chun-Yan Lu1, Lei Dong1, Shuai Li1
1State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China.
Current Microbiology
|March 17, 2023
Summary
A novel actinobacterium, Salinibacterium sedimenticola SYSU T00001T, was isolated from Chinese tidal flats. This new species expands the known diversity within the Salinibacterium genus.
Area of Science:
- Microbiology
- Bacteriology
- Taxonomy
Background:
- Actinobacteria are crucial microorganisms in various environments.
- Tidal flat sediments are rich sources of novel microbial diversity.
- The genus Salinibacterium comprises halophilic bacteria with unique adaptations.
Purpose of the Study:
- To isolate and characterize a novel actinobacterium from a tidal flat sediment.
- To determine the taxonomic position of the novel isolate using polyphasic characterization, including whole genome sequencing.
- To propose a new species within the genus Salinibacterium.
Main Methods:
- Isolation and cultivation of bacteria from tidal flat sediment.
- Phenotypic characterization including morphology, growth conditions, and biochemical tests.
- Chemotaxonomic analyses (menaquinones, fatty acids, polar lipids, cell wall composition).
- Genomic analyses including whole genome sequencing and phylogenetic analysis (16S rRNA gene, ANI, dDDH).
Main Results:
- A novel Gram-stain-positive, aerobic, motile actinobacterium, strain SYSU T00001T, was isolated.
- The isolate exhibited tolerance to a wide range of temperatures, pH, and NaCl concentrations.
- Phylogenetic analyses based on 16S rRNA gene sequences and whole-genome data placed the strain within the genus Salinibacterium, with highest similarity to Salinibacterium hongtaonis 194T (97.8%).
- Low ANI (74.5%) and dDDH (19.5%) values supported its classification as a distinct species.
Conclusions:
- Strain SYSU T00001T represents a novel species of the genus Salinibacterium.
- The proposed name for this new species is Salinibacterium sedimenticola sp. nov.
- The type strain is SYSU T00001T (GDMCC 1.3283T = KCTC 49758T).
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