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Updated: Aug 6, 2025

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A Novel Approach to Monitoring Graft Neovascularization in the Human Gingiva
Published on: January 12, 2019
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Gingival phenotype changes after different periodontal plastic surgical techniques: a single-masked randomized
Pasquale Santamaria1,2, Michele Paolantonio2, Luigi Romano2
1Periodontology Unit, Centre for Host Microbiome Interactions, Faculty of Dentistry, Oral & Craniofacial Sciences, King's College London, London, UK.
Clinical Oral Investigations
|March 17, 2023
Summary
The coronally advanced flap with sub-epithelial connective tissue graft (SCTG) offers the most predictable increase in gingival thickness and keratinized tissue width for root coverage. This technique enhances gingival phenotype for long-term stability.
Area of Science:
- Periodontology
- Oral Surgery
- Regenerative Dentistry
Background:
- Gingival recession affects oral health and aesthetics.
- Root coverage procedures aim to restore lost gingival tissue.
- Comparing surgical techniques for root coverage is crucial for clinical decision-making.
Purpose of the Study:
- To evaluate the effects of three surgical techniques on gingival thickness for root coverage.
- To compare coronally advanced flap (CAF) alone, CAF with sub-epithelial connective tissue graft (SCTG), and CAF with leukocyte- and platelet-rich fibrin (L-PRF) membranes.
Main Methods:
- Sixty patients with RT1 single maxillary gingival recession were randomized into three groups (CAF+L-PRF, CAF+SCTG, CAF alone).
- Gingival thickness (GT), keratinized tissue width (KT), gingival recession (GR), and clinical attachment level (CAL) were assessed at baseline and 6 months.
- Aesthetic outcomes and patient-reported measures were also recorded.
Main Results:
- CAF+SCTG and CAF+L-PRF significantly increased GT compared to CAF alone.
- CAF+SCTG showed a significantly greater increase in KT compared to the other groups.
- All techniques resulted in significant GR reduction and CAL gain, with no significant differences between groups for aesthetic outcomes.
Conclusions:
- All techniques effectively reduced gingival recession and improved clinical attachment levels.
- CAF+SCTG and CAF+L-PRF similarly increased gingival thickness.
- CAF+SCTG demonstrated the most predictable increase in keratinized tissue and gingival thickness, suggesting it is optimal for improving gingival phenotype and stability.

