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Two B-box proteins, PavBBX6/9, positively regulate light-induced anthocyanin accumulation in sweet cherry
Yanyan Wang1, Yuqin Xiao1, Yueting Sun1
1Department of Pomology, College of Horticulture, China Agricultural University, Beijing, 100193, China.
Plant Physiology
|March 17, 2023
Summary
Light exposure induces red color in sweet cherries by increasing anthocyanin and abscisic acid (ABA) production. Two B-box genes, PavBBX6/9, are key regulators, promoting both anthocyanin and ABA biosynthesis pathways.
Area of Science:
- Plant Biology
- Molecular Biology
- Agricultural Science
Background:
- Light exposure is crucial for anthocyanin production and red coloration in bicolored sweet cherry (Prunus avium cv. Rainier) fruit.
- Abscisic acid (ABA) is essential for this process, but the precise regulatory links between light, ABA, and anthocyanin accumulation remain unclear.
Purpose of the Study:
- To elucidate the regulatory mechanisms connecting light, ABA, and anthocyanin biosynthesis in sweet cherry fruit.
- To investigate the role of specific B-box (BBX) genes in mediating these light-induced responses.
Main Methods:
- Analyzing anthocyanin and ABA levels in response to light and ABA treatments in sweet cherry fruit.
- Ectopic expression of PavBBX6/9 in Arabidopsis and sweet cherry calli to assess their effects on anthocyanin and ABA accumulation.
- Transient overexpression and gene silencing of PavBBX6/9 in sweet cherry peel.
- Promoter analysis to determine the binding of PavBBX6/9 to key biosynthetic genes (PavUFGT and PavNCED1).
Main Results:
- Light treatment significantly increased anthocyanin and ABA accumulation in sweet cherry fruit.
- Ectopic expression and overexpression of PavBBX6/9 enhanced both anthocyanin and ABA biosynthesis.
- Silencing PavBBX6/9 reduced anthocyanin and ABA levels.
- PavBBX6/9 directly bind to and activate the promoters of PavUFGT (anthocyanin biosynthesis) and PavNCED1 (ABA biosynthesis).
Conclusions:
- PavBBX6 and PavBBX9 are positive regulators of light-induced anthocyanin and ABA biosynthesis in sweet cherry fruit.
- These BBX genes act by promoting the expression of key genes in both pathways, namely PavUFGT and PavNCED1.
- This study clarifies the intricate relationship between light signaling, ABA biosynthesis, and anthocyanin accumulation in fruit coloration.
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