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Related Concept Videos

Zygotic Development And Stem Cell Formation01:10

Zygotic Development And Stem Cell Formation

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The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...
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Cleavage and Blastulation01:33

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After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.
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Related Experiment Video

Updated: Aug 6, 2025

Derivation of Mouse Trophoblast Stem Cells from Blastocysts
10:19

Derivation of Mouse Trophoblast Stem Cells from Blastocysts

Published on: June 8, 2010

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Protocol for capturing trophectoderm stem cells reflecting the blastocyst stage.

Jinwoo Seong1, Nicolas C Rivron1

  • 1Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna Biocenter, 1030 Vienna, Austria.

STAR Protocols
|March 17, 2023
PubMed
Summary
This summary is machine-generated.

A refined combination of growth factors enhances the derivation of trophectoderm stem cells from mouse blastocysts. This improved method yields cells with greater similarity to early development and better self-renewal capabilities.

Keywords:
Biotechnology and BioengineeringDevelopmental BiologyStem CellsTissue Engineering

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Last Updated: Aug 6, 2025

Derivation of Mouse Trophoblast Stem Cells from Blastocysts
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Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells
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Area of Science:

  • Developmental Biology
  • Stem Cell Biology

Background:

  • Trophoblast stem cells (TSCs) are crucial for placental development.
  • Classical methods using FGF4/TGF-β1 yield TSCs with mixed subpopulations.

Purpose of the Study:

  • To improve the derivation and characteristics of TSCs.
  • To enhance the developmental potential of derived TSCs.

Main Methods:

  • Culturing mouse blastocysts with a comprehensive set of inducers: FGF4, TGF-β1, LPA, IL11, BMP7, Activin A, and 8-Br-cAMP.
  • Transcriptomic analysis to compare derived TSCs with blastocyst trophectoderm.

Main Results:

  • A complete combination of inducers generated trophectoderm stem cells with enhanced transcriptomic similarity to blastocyst trophectoderm.
  • These cells exhibited improved self-renewal and reduced differentiation.
  • The complete inducer combination increased the potential for blastoid formation and in utero decidualization.

Conclusions:

  • The comprehensive inducer cocktail provides a superior method for deriving TSCs.
  • These enhanced TSCs more accurately model early embryonic development and possess greater self-renewal capacity.