Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Metabolism01:19

Regulation of Metabolism

9.6K
Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...
9.6K
Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

3.2K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
3.2K
Mitochondrial Membranes01:45

Mitochondrial Membranes

11.7K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
11.7K
Introduction to Metabolism01:30

Introduction to Metabolism

167
Metabolism encompasses all biochemical reactions in a living organism, facilitating both the breakdown and synthesis of biomolecules. These metabolic processes are categorized into catabolic and anabolic pathways, which operate in a coordinated manner to ensure energy balance and cellular function.Catabolic Pathways and Energy ReleaseCatabolic pathways involve the breakdown of complex macromolecules such as carbohydrates, lipids, and proteins into smaller structures like monosaccharides, fatty...
167
The Inner Mitochondrial Membrane01:28

The Inner Mitochondrial Membrane

3.5K
The inner mitochondrial membrane is the primary site of ATP synthesis. The inner membrane domain that forms a smooth layer adjacent to the outer membrane is called the inner boundary membrane. This domain contains membrane transporters that drive metabolites in and out of the mitochondria.  In contrast, the inner membrane network that invaginates into the matrix space is called the cristae membrane. This domain accounts for principle mitochondrial function as it accommodates the protein...
3.5K
Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

14.7K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
14.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

LSCA-RCNN: Large-Kernel Spatial Residual and Cascade Attention Network for Voxel-Based 3D Object Detection.

Sensors (Basel, Switzerland)·2026
Same author

Navigating chemical-linguistic sharing space with heterogeneous molecular encoding.

Nature communications·2026
Same author

Gene expression profiling enables refined parcellation of cortical layers in the heterogeneous human cerebral cortex.

Genome medicine·2026
Same author

Rapid-access oncodermatology is associated with reduced corticosteroid use and supports continuation of anticancer therapy after treatment-related cutaneous toxicity.

JAAD international·2026
Same author

Advances in Calibration Methods for FDR-Based Capacitive Soil Moisture Sensors.

Sensors (Basel, Switzerland)·2026
Same author

Pan-cancer analysis and mechanistic insights of tuft cell-like tumors.

Genes & diseases·2026

Related Experiment Video

Updated: Aug 6, 2025

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells
12:22

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells

Published on: July 22, 2013

21.1K

Metabolic sensing and control in mitochondria.

Yuyang Liu1, Kıvanç Birsoy1

  • 1Laboratory of Metabolic Regulation and Genetics, The Rockefeller University, New York, NY, USA.

Molecular Cell
|March 17, 2023
PubMed
Summary

Mitochondria sense and control metabolites to maintain cellular health and respond to stress. Understanding these regulatory circuits is key to addressing metabolic disorders.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Metabolic Regulation

Background:

  • Mitochondria, with their own genomes, are crucial for cellular energy and metabolism.
  • They must coordinate with cytosolic metabolism, requiring sophisticated sensing and control mechanisms.
  • Dysfunctional mitochondrial regulation leads to inborn errors of metabolism.

Purpose of the Study:

  • To review the logic and molecular mechanisms of metabolic sensing and control in mitochondria.
  • To identify recurring patterns and knowledge gaps in mitochondrial metabolic regulation.
  • To highlight how emerging technologies can address these gaps.

Main Methods:

  • Literature review of existing research on mitochondrial metabolic control.
  • Analysis of regulatory circuits and metabolic sensors.

More Related Videos

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
08:57

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases

Published on: February 24, 2018

10.1K
Author Spotlight: An Optimized Automated Method for Investigating Retinoic Acid Receptors in Neuronal Mitochondria
08:33

Author Spotlight: An Optimized Automated Method for Investigating Retinoic Acid Receptors in Neuronal Mitochondria

Published on: July 28, 2023

655

Related Experiment Videos

Last Updated: Aug 6, 2025

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells
12:22

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells

Published on: July 22, 2013

21.1K
Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
08:57

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases

Published on: February 24, 2018

10.1K
Author Spotlight: An Optimized Automated Method for Investigating Retinoic Acid Receptors in Neuronal Mitochondria
08:33

Author Spotlight: An Optimized Automated Method for Investigating Retinoic Acid Receptors in Neuronal Mitochondria

Published on: July 28, 2023

655
  • Discussion of emerging technologies relevant to mitochondrial research.

    • Main Results:

    • Mitochondria employ feedback and feedforward strategies for metabolic control.
    • Diverse metabolic sensors mediate these regulatory circuits.
    • Recurring patterns in mitochondrial metabolic sensing and control have been identified.

    Conclusions:

    • Understanding mitochondrial metabolic control is vital for comprehending cellular homeostasis.
    • Further research, aided by new technologies, is needed to fill knowledge gaps in mitochondrial regulation.
    • This knowledge is critical for developing treatments for metabolic diseases.