Generating Aedes aegypti Mutant Strains with Transgenic Cas9

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Ruichen Sun1, Robyn Raban1, Omar S Akbari2

  • 1Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, California 92093, USA.

Cold Spring Harbor Protocols
|March 17, 2023
PubMed

Abstract:

Here, we provide a protocol for generating Aedes aegypti mutant strains via end-joining (EJ) or homology-directed repair (HDR) mechanisms using genetically encoded Cas9.

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