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Generating Aedes aegypti Mutant Strains with Transgenic Cas9
Ruichen Sun1, Robyn Raban1, Omar S Akbari2
1Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, California 92093, USA.
Cold Spring Harbor Protocols
|March 17, 2023
Abstract:
Here, we provide a protocol for generating Aedes aegypti mutant strains via end-joining (EJ) or homology-directed repair (HDR) mechanisms using genetically encoded Cas9.
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