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Related Experiment Videos

Lung endothelial and epithelial permeability after platelet-activating factor.

R W Bolin1, T R Martin, R K Albert

  • 1Medical Service, Veterans Administration Medical Center, Seattle, Washington.

Journal of Applied Physiology (Bethesda, Md. : 1985)
|November 1, 1987
PubMed
Summary

Platelet-activating factor (PAF) does not increase lung epithelial or endothelial permeability. PAF administration in isolated rabbit lungs elevated intravascular pressure but did not alter lung fluid filtration, lavage protein concentration, or wet-to-dry weight ratios.

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Area of Science:

  • Pulmonary physiology
  • Vascular biology
  • Inflammation research

Background:

  • Platelet-activating factor (PAF) is a potent mediator implicated in inflammatory processes.
  • Understanding PAF's role in vascular permeability is crucial for respiratory medicine.

Purpose of the Study:

  • To determine if platelet-activating factor (PAF) increases epithelial or endothelial permeability in isolated-perfused rabbit lungs.
  • To investigate the influence of neutrophils and platelets on PAF-induced changes in lung permeability.

Main Methods:

  • Isolated-perfused rabbit lungs were used to assess vascular permeability.
  • PAF was administered via the pulmonary artery or airway.
  • Fluid filtration coefficient (Kf), bronchoalveolar lavage protein (BALP), and wet-to-dry weight ratios (W/D) were measured.

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  • The effects of neutrophils and platelets on PAF-induced responses were evaluated.

    • Main Results:

    • PAF administration into the vascular space increased pulmonary arterial pressure (Ppa), an effect amplified by platelets.
    • PAF did not significantly increase Ppa when instilled into the airway.
    • Neither vascular nor airway administration of PAF altered Kf, W/D, or BALP, indicating no increase in epithelial or endothelial permeability.
    • The presence of neutrophils or platelets did not modify the lack of permeability increase.

    Conclusions:

    • Platelet-activating factor (PAF) increases intravascular pressure in isolated rabbit lungs but does not induce epithelial or endothelial permeability.
    • Neutrophils and platelets do not enhance PAF-induced lung permeability in this model.
    • These findings clarify the specific effects of PAF on lung vasculature, distinguishing pressure changes from permeability alterations.