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Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
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Proteomics and transcriptomics profiling reveals distinct aspects of kidney stone related genes in calculi rats

Wang Zhu1, Deng Qiong1, Gu Yanli2

  • 1Department of Urology, People's Hospital of Longhua Shenzhen, Southern Medical University, 38 Jinglong Jianshe Road, Shenzhen, Guangdong, 518109, People's Republic of China.

BMC Genomics
|March 18, 2023
PubMed
Abstract

Insights

This study reveals key gene expression changes in kidney stones (nephrolithiasis) using proteomic and transcriptomic analysis. Findings offer insights into disease mechanisms and potential prevention strategies.

Area of Science:

  • Genomics and Proteomics
  • Nephrology
  • Molecular Biology

Background:

  • Kidney stones (urolithiasis/nephrolithiasis) are ancient but their molecular underpinnings remain poorly understood.
  • Gene expression changes and associated kidney injury mechanisms require further elucidation.

Purpose of the Study:

  • To characterize distinct gene expression profiles in kidney stones using integrated analysis.
  • To identify key molecular pathways involved in nephrolithiasis pathogenesis.

Main Methods:

  • Developed a rat model of kidney stones induced by ethylene glycol and ammonium chloride.
  • Performed integrated proteomic and transcriptomic analysis to identify differentially expressed genes (DEGs).
  • Utilized Gene Ontology and KEGG pathway analysis for functional enrichment and immunohistochemistry for validation.

Main Results:

  • Quantified 5,897 genes at both transcriptome and proteome levels, identifying six gene clusters with 14 consistently dysregulated genes.
  • Observed increased expression of injury/apoptotic markers and immune molecules.
  • Noted decreased expression of solute carriers, transporters, and metabolic factors in calculi rat kidneys.

Conclusions:

  • The proteotranscriptomic data provides a valuable resource for understanding nephrolithiasis.
  • Findings offer new insights into the pathogenesis of kidney stone disease.
  • This research may facilitate the development of novel strategies for kidney stone recurrence prevention and treatment.
Keywords:
Calcium oxalateKidney stoneProteomicsTranscriptomicsUrolithiasis

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