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Updated: Jul 21, 2026

Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals
Published on: January 5, 2016
Plasma extracellular vesicles and cell-free mitochondrial DNA are associated with cognitive dysfunction in treated
Carrie D Johnston1, Erika G Marques de Menezes2,3,4, Scott Bowler5
1Division of Infectious Diseases, Weill Cornell Medicine, 525 E. 68th St, Baker Tower, F2331, NY, 10065, New York, USA. cmd9008@med.cornell.edu.
Abstract:
Extracellular vesicles (EVs) are nanoparticles with a role in intercellular communication. Cell-free mitochondrial DNA (cf-mtDNA) has been associated with cognitive dysfunction in people with HIV (PWH). We conducted a nested case-control study to test the hypothesis that plasma EVs are associated with cf-mtDNA and cognitive dysfunction in older PWH. A machine learning-based model identified total EVs, including select EV subpopulations, as well as urine cf-mtDNA and 4-meter walk time carry power to predict the neurocognitive impairment. These features resulted in an AUC-ROC of 0.845 + / - 0.109 (0.615, 1.00).
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