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Area of Science:

  • Oncology
  • Medical Imaging
  • Surgical Technology

Background:

  • Fluorescence-guided surgery (FGS) is crucial for pediatric tumor management.
  • Shortwave infrared (SWIR) imaging offers advantages over near-infrared I (NIR-I) due to reduced scattering and autofluorescence.
  • Targeting GD2-expressing neuroblastoma with antibodies is a key therapeutic strategy.

Purpose of the Study:

  • To compare NIR-I and SWIR imaging for neuroblastoma surgery using GD2-targeted dyes.
  • To develop and validate a multispectral NIR-I/SWIR fluorescence imaging device.
  • To assess the sensitivity, depth penetration, and tumor-to-background ratio (TBR) of the system.

Main Methods:

  • Conjugation of NIR-I dyes (IRDye800CW, IR12) to anti-GD2 antibody (dinutuximab-beta).
  • In vitro characterization and phantom studies to evaluate sensitivity and depth penetration (up to 3 mm).
  • In vivo fluorescence imaging using a custom-built multispectral NIR-I/SWIR device in a neuroblastoma model.

Main Results:

  • The multispectral device achieved a minimum detectable volume of ~0.9 mm³.
  • Both NIR-I and SWIR imaging demonstrated high TBR, with anti-GD2-IR800 showing higher brightness.
  • SWIR imaging consistently yielded higher TBR than NIR-I, enabling superior tumor margin delineation.

Conclusions:

  • GD2-targeted SWIR fluorescence imaging provides high-contrast visualization of neuroblastoma tumors.
  • This technology combines antibody specificity with SWIR imaging advantages for improved surgical guidance.
  • Further clinical trials are warranted to investigate GD2-targeted NIR-I/SWIR surgery for neuroblastoma treatment.