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Updated: Aug 6, 2025
Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer SALB Method
Published on: December 1, 2015
Label-free and selective cholesterol detection based on multilayer functional structure coated fiber fabry-perot
Ruiduo Wang1, Minglu Yan2, Man Jiang2
1State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an, 710119, People's Republic of China; State Key Laboratory of Photon-Technology in Western China Energy, International Collaborative Center on Photoelectric Technology and Nano Functional Materials, School of Physics, Institute of Photonics&Photon Technology, Northwest University, Xi'an, 710069, People's Republic of China.
This study introduces a novel fiber-optic sensor for cholesterol detection. The sensor utilizes a multi-layer film for high sensitivity and selectivity, offering a promising platform for biosensing applications.
Area of Science:
- Optoelectronics
- Biomedical Engineering
- Materials Science
Background:
- Cholesterol measurement is crucial for diagnosing cardiovascular diseases.
- Existing methods for cholesterol detection can be complex and time-consuming.
- Development of sensitive and selective biosensors is an ongoing research area.
Purpose of the Study:
- To develop a novel reflective fiber-optic Fabry-Perot cavity probe sensor for selective cholesterol concentration measurement.
- To immobilize a multi-layer film of epoxy resin (ER)/graphene oxide (GO)/beta-cyclodextrin (β-CD) onto a ceramic tube end face for enhanced sensing capabilities.
Main Methods:
- Fabrication of a fiber-optic sensor by inserting a single-mode fiber into a ceramic tube.
- Immobilization of an epoxy resin (ER)/graphene oxide (GO)/beta-cyclodextrin (β-CD) multi-layer film onto the ceramic tube end face.
- Utilizing EDC/NHS activated GO for chemical binding with β-CD, which acts as the cholesterol-binding sensitive material.
Main Results:
- The developed sensor achieved a sensitivity of 3.92 nm/mM for cholesterol concentration.
- The limit of detection for cholesterol was determined to be 3.48 μM.
- The sensor demonstrated high selectivity, successfully distinguishing cholesterol in the presence of hemoglobin, glucose, and ascorbic acid, with high reproducibility and reusability.
Conclusions:
- The proposed fiber-optic Fabry-Perot cavity probe sensor offers a sensitive and selective method for cholesterol measurement.
- The sensor exhibits excellent mechanical strength, ease of fabrication, real-time monitoring capabilities, low cost, and user-friendliness.
- This sensor presents a remarkable analytical platform for various biosensing applications.
