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Urease bound to chitin with glutaraldehyde.

L Iyengar, A V Rao

    Biotechnology and Bioengineering
    |August 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Urease enzyme from Cajanus indicus was immobilized on glutaraldehyde-treated chitin. This study characterized the enzyme

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    Area of Science:

    • Biochemistry
    • Enzyme Technology
    • Biomaterials

    Background:

    • Urease enzyme (urea amidohydrolase, EC 3.5.1.5) is crucial in various biological and industrial processes.
    • Immobilization of enzymes enhances their stability and reusability.
    • Chitin is a biocompatible and abundant natural polymer suitable for enzyme support.

    Purpose of the Study:

    • To immobilize urease from Cajanus indicus onto glutaraldehyde-treated chitin.
    • To characterize the biochemical properties of the immobilized urease.
    • To evaluate the stability and optimal conditions for the immobilized enzyme system.

    Main Methods:

    • Enzyme immobilization using glutaraldehyde-activated chitin as a solid support.
    • Characterization of immobilized urease, including pH profiles and optimal temperature determination.

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  • Investigation of glutaraldehyde concentration's effect on enzyme binding and assessment of storage stability.

    • Main Results:

    • Successful immobilization of urease onto glutaraldehyde-treated chitin was achieved.
    • The optimal pH and temperature for the immobilized urease were determined.
    • The study elucidated the impact of glutaraldehyde concentration on enzyme-support interaction and assessed the system's storage stability.

    Conclusions:

    • Glutaraldehyde-treated chitin is an effective support for urease immobilization.
    • The immobilized urease system exhibits favorable characteristics for potential applications.
    • Further research can explore the practical utility of this immobilized enzyme in various fields.