Related Experiment Video
Updated: Aug 6, 2025
10:34
Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
Published on: December 9, 2022
4.3K
How well does a data-driven prediction method distinguish dihydrouridine from tRNA and mRNA?
Shaherin Basith1, Balachandran Manavalan2
1Department of Physiology, Ajou University School of Medicine, Suwon 16499, Republic of Korea.
Molecular Therapy. Nucleic Acids
|March 20, 2023
Summary
No abstract available in PubMed .
Related Concept Videos
02:07Improving Translational Accuracy
11.7K
Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
11.7K
02:26tRNA Activation
19.5K
Aminoacyl-tRNA synthetases are present in both eukaryotes and bacteria. Though eukaryotes have 20 different aminoacyl-tRNA synthetases to couple to 20 amino acids, many bacteria do not have genes for all of these aminoacyl-tRNA synthetases. Despite this, they still use all 20 amino acids to synthesize their proteins. For instance, some bacteria do not have the gene encoding the enzyme that couples glutamine with its partner tRNA. In these organisms, one enzyme adds glutamic acid to all of the...
19.5K