Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Anchoring Junctions01:03

Anchoring Junctions

3.9K
Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
3.9K
RC Circuit without Source01:16

RC Circuit without Source

1.2K
When a DC source is abruptly disconnected from an RC (Resistor-Capacitor) circuit, the circuit becomes source-free. Assuming that the capacitor was fully charged before the source was removed, its initial voltage, denoted as V0, can be considered as the initial energy that stimulates the circuit.
Applying Kirchhoff's current law at the top node of the circuit and substituting the current values across the components, a first-order differential equation is obtained. By rearranging the terms...
1.2K
RL Circuit without Source01:14

RL Circuit without Source

981
When a DC source is suddenly disconnected from an RL (Resistor-Inductor) circuit, the circuit becomes source-free. Assuming the inductor has an initial current denoted as I0, the initial energy stored in the inductor can be determined.
Applying Kirchhoff's voltage law around the loop of the circuit and substituting the voltages across the inductor and resistor yields a first-order differential equation. A logarithmic equation is obtained by rearranging the terms in this equation,...
981
RC Circuit with Source01:15

RC Circuit with Source

1.2K
When a DC source is abruptly applied to an RC (Resistor-Capacitor) circuit, the voltage can be represented as a unit step function. The voltage across the capacitor, known as the step response, characterizes how the circuit reacts to this sudden change in input.
Due to the inherent properties of a capacitor, its voltage cannot change instantaneously. This means that immediately after the switch is closed, the capacitor's voltage remains the same as it was just before the switch was closed.
1.2K
Source Transformation01:15

Source Transformation

7.0K
Source transformation is a fundamental technique employed in circuit analysis, offering a valuable tool for simplifying complex electrical circuits. This technique involves the replacement of either a voltage source in series with a resistor by a current source in parallel with a resistor, or vice versa. The key concept here is that when the original sources are deactivated (turned off), the equivalent resistance at the circuit's end terminals remains the same.
It is essential to note that when...
7.0K
Underflow Gates01:30

Underflow Gates

93
Underflow gates are vital for controlling water flow in irrigation canals. The three main types of underflow gates — vertical, radial, and drum gates — serve different purposes while ensuring effective flow management. Vertical gates move up and down, generating a free-flowing water jet; radial gates pivot to regulate the flow; and drum gates rotate for precise adjustments. The flow through these gates is influenced by downstream conditions, resulting in free or drowned outflow.Free and...
93

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metabolic control of smooth muscle cell phenotype switching in atherosclerosis.

bioRxiv : the preprint server for biology·2026
Same author

Vascular Endothelial Growth Factor-D Improves Lung Vascular Integrity During Acute Lung Injury.

Circulation research·2026
Same author

TWIST1 drives endothelial-to-mesenchymal-transition to stabilize atherosclerotic plaques.

Nature communications·2026
Same author

Retraction Note: SUMOylation of VEGFR2 regulates its intracellular trafficking and pathological angiogenesis.

Nature communications·2026
Same author

A Novel Application-Based Test for Rapid Screening of Olfactory Dysfunction.

JAMA otolaryngology-- head & neck surgery·2026
Same author

A fibroblast-like endothelial cell state promotes atherosclerosis via C/EBPβ-activated TGF-β signaling.

The EMBO journal·2026
Same journal

Loss of STMP1 impairs mitochondrial structure and links inflammaging to heart failure.

Nature cardiovascular research·2026
Same journal

HeartMap charts new territory in cardiac disease biology.

Nature cardiovascular research·2026
Same journal

An integrated cell atlas of 2.4 million cardiac cells across 209 individuals in health and disease.

Nature cardiovascular research·2026
Same journal

Interim results of the Heart-2 clinical trial testing the safety and efficacy of VERVE-102 therapy.

Nature cardiovascular research·2026
Same journal

Publisher Correction: Genetic perspectives on the comorbidity of anxiety and mood disorders with cardiovascular disease.

Nature cardiovascular research·2026
Same journal

Author Correction: Ventricular assist device unloading reverses microvascular senescence in single ventricle disease.

Nature cardiovascular research·2026
See all related articles

Related Experiment Video

Updated: Aug 6, 2025

Cut-loading: A Useful Tool for Examining the Extent of Gap Junction Tracer Coupling Between Retinal Neurons
10:11

Cut-loading: A Useful Tool for Examining the Extent of Gap Junction Tracer Coupling Between Retinal Neurons

Published on: January 12, 2012

15.5K

YES to Junctions, No to Src.

Michael Simons1, Derek Toomre1

  • 1Yale Cardiovascular Research Center and Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06511.

Nature Cardiovascular Research
|March 20, 2023
PubMed
Summary
This summary is machine-generated.

Endothelial barrier function is vital for blood vessel health. A new study reveals how VE-cadherin phosphorylation, involving YES, regulates vascular permeability and integrity.

More Related Videos

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.0K
A Functional Assay for Gap Junctional Examination; Electroporation of Adherent Cells on Indium-Tin Oxide
11:02

A Functional Assay for Gap Junctional Examination; Electroporation of Adherent Cells on Indium-Tin Oxide

Published on: October 18, 2014

10.0K

Related Experiment Videos

Last Updated: Aug 6, 2025

Cut-loading: A Useful Tool for Examining the Extent of Gap Junction Tracer Coupling Between Retinal Neurons
10:11

Cut-loading: A Useful Tool for Examining the Extent of Gap Junction Tracer Coupling Between Retinal Neurons

Published on: January 12, 2012

15.5K
Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.0K
A Functional Assay for Gap Junctional Examination; Electroporation of Adherent Cells on Indium-Tin Oxide
11:02

A Functional Assay for Gap Junctional Examination; Electroporation of Adherent Cells on Indium-Tin Oxide

Published on: October 18, 2014

10.0K

Area of Science:

  • Vascular biology
  • Cellular signaling
  • Physiology

Background:

  • Endothelial barrier integrity is crucial for vascular homeostasis.
  • Dynamic regulation of endothelial permeability is essential in various physiological and pathological conditions.
  • VE-cadherin phosphorylation is a key mechanism controlling endothelial cell-cell junctions.

Purpose of the Study:

  • To elucidate the intricate relationship between VE-cadherin phosphorylation and vascular leak.
  • To investigate the specific role of the YES kinase in regulating endothelial barrier function.
  • To understand the molecular mechanisms underlying vascular permeability.

Main Methods:

  • Western blotting to assess protein phosphorylation.
  • Immunofluorescence microscopy to visualize VE-cadherin localization.
  • Endothelial cell culture models to study barrier function.
  • In vivo models to assess vascular leak.

Main Results:

  • VE-cadherin phosphorylation was identified as a critical regulator of endothelial barrier function.
  • The study highlights the pivotal role of YES kinase in mediating VE-cadherin phosphorylation.
  • Increased VE-cadherin phosphorylation correlated with enhanced vascular permeability and leak.

Conclusions:

  • YES-mediated VE-cadherin phosphorylation is a key determinant of vascular leak.
  • Targeting this pathway may offer therapeutic strategies for vascular permeability disorders.
  • Understanding these molecular events is crucial for maintaining vascular health.