Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gene Regulation During Sporulation01:17

Gene Regulation During Sporulation

45
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
45
Autophagy01:27

Autophagy

4.4K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
4.4K
Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

6.6K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
6.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The efficacy-invasiveness trade-off: a retrospective cohort comparison of surgical operation and endobronchial valves for refractory lung bullae disease.

BMC pulmonary medicine·2026
Same author

Evolutionary diversification of the <i>STAYGREEN</i> gene family in <i>Nicotiana</i>.

Frontiers in plant science·2026
Same author

Recent advances in capsanthin: Sources, bioactivities, and potential strategies for utilization.

Food chemistry·2026
Same author

An engineered bacteriocin paracin wx7-1 for chili pepper preservation: Controlling soft rot and its dual-target mechanisms.

International journal of food microbiology·2026
Same author

Development of a CT-based integrated model combining clinical features, radiomics, and deep learning to predict STAS in subsolid nodules of stage I lung cancer: a retrospective cohort study.

Journal of thoracic disease·2026
Same author

Revolutionizing lung cancer screening: the rise of artificial intelligence integrating circulating tumor markers.

World journal of surgical oncology·2026

Related Experiment Video

Updated: Aug 6, 2025

An Efficient Clearing Protocol for the Study of Seed Development in Tomato Solanum lycopersicum L.
06:26

An Efficient Clearing Protocol for the Study of Seed Development in Tomato Solanum lycopersicum L.

Published on: September 7, 2022

4.3K

SlMYB72 affects pollen development by regulating autophagy in tomato.

Mengbo Wu1,2, Qiongdan Zhang1,2, Guanle Wu1,2

  • 1Key Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing University, Chongqing 400044, China.

Horticulture Research
|March 20, 2023
PubMed
Summary
This summary is machine-generated.

SlMYB72 is crucial for tomato pollen development and seed production. This gene regulates tapetum degradation and autophagy by activating SlATG7, impacting plant reproduction and offering insights into male sterility.

More Related Videos

Author Spotlight: Advancing Stomatal Research with Automated Aperture Measurement
05:03

Author Spotlight: Advancing Stomatal Research with Automated Aperture Measurement

Published on: February 9, 2024

1.7K
Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System
08:54

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

Published on: March 20, 2016

9.9K

Related Experiment Videos

Last Updated: Aug 6, 2025

An Efficient Clearing Protocol for the Study of Seed Development in Tomato Solanum lycopersicum L.
06:26

An Efficient Clearing Protocol for the Study of Seed Development in Tomato Solanum lycopersicum L.

Published on: September 7, 2022

4.3K
Author Spotlight: Advancing Stomatal Research with Automated Aperture Measurement
05:03

Author Spotlight: Advancing Stomatal Research with Automated Aperture Measurement

Published on: February 9, 2024

1.7K
Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System
08:54

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

Published on: March 20, 2016

9.9K

Area of Science:

  • Plant Biology
  • Molecular Genetics
  • Reproductive Biology

Background:

  • Pollen formation and development are vital for plant reproduction and genetic diversity.
  • The tapetum layer of the anther plays a critical role in providing nutrients for pollen development.

Purpose of the Study:

  • To investigate the role of SlMYB72 in tomato pollen and tapetum development.
  • To elucidate the molecular mechanism by which SlMYB72 regulates these processes.

Main Methods:

  • Gene expression analysis of SlMYB72 in tomato anthers.
  • RNA interference (RNAi) to downregulate SlMYB72 and assess effects on pollen and tapetum.
  • Overexpression of SlMYB72 to study its impact.
  • Analysis of autophagy-related genes (ATGs) and tapetum degradation.
  • Promoter analysis to identify SlMYB72 binding sites and its effect on SlATG7 expression.

Main Results:

  • SlMYB72 is highly expressed in tomato pollen and tapetum.
  • Downregulation of SlMYB72 resulted in abnormal pollen development, reduced seed set, delayed tapetum degradation, and inhibited autophagy.
  • Overexpression of SlMYB72 also led to abnormal pollen development and delayed tapetum degradation.
  • SlMYB72 directly activates the expression of SlATG7, a key autophagy gene.
  • Downregulation of SlATG7 mimicked the effects of SlMYB72 downregulation on autophagy and pollen development.

Conclusions:

  • SlMYB72 regulates tapetum degradation and pollen development through the transcriptional activation of SlATG7 and modulation of autophagy in tomato anthers.
  • Autophagy is critical for proper pollen development in tomatoes.
  • SlMYB72 and SlATG7 are potential targets for manipulating male sterility in plants.