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Standardized Methods for Measuring Induction of the Heat Shock Response in Caenorhabditis elegans
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The class B heat shock factor HSFB1 regulates heat tolerance in grapevine
Haiyang Chen1,2,3, Xinna Liu1,2,3, Shenchang Li1,2,3
1Beijing Key Laboratory of Grape Science and Enology and Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
Horticulture Research
|March 20, 2023
Summary
Heat Shock Factor B1 (HSFB1) gene enhances heat tolerance in grapevines. Differences in HSFB1 promoter activity explain varying heat tolerance between grape cultivars, aiding in breeding efforts for heat-resistant grapes.
Area of Science:
- Plant Molecular Biology
- Plant Physiology
- Genetics and Genomics
Background:
- Grape cultivation faces challenges from increasing heat stress, impacting yield and quality.
- Understanding the genetic mechanisms of heat tolerance is crucial for developing resilient grape varieties.
- Existing cultivars often lack sufficient tolerance to elevated temperatures.
Purpose of the Study:
- To investigate the molecular mechanisms underlying heat tolerance in grapevines.
- To identify key genes and regulatory elements involved in thermotolerance.
- To provide insights for breeding heat-tolerant grape cultivars.
Main Methods:
- Comparative transcriptome analysis of heat-tolerant (Vitis davidii 'Tangwei') and heat-sensitive (Vitis vinifera 'Jingxiu') grapevines under heat stress.
- Identification and characterization of differentially expressed genes (DEGs), focusing on Heat Shock Factor B1 (HSFB1).
- Gene expression analysis, promoter activity assays, and genetic manipulation (overexpression and RNA interference) in grapevines.
Main Results:
- Significantly more DEGs were observed in the heat-tolerant 'Tangwei' compared to 'Jingxiu' under heat stress.
- HSFB1 was significantly upregulated in 'Tangwei' but not in 'Jingxiu' at high temperatures.
- HSFB1 positively regulates heat tolerance, with higher promoter activity and more TATA-box elements in the heat-tolerant 'Tangwei' cultivar.
Conclusions:
- HSFB1 plays a critical role in conferring heat tolerance in grapevines.
- Variations in HSFB1 promoter activity, influenced by cis-elements, contribute to differential thermotolerance between grape germplasms.
- This study provides a foundation for breeding heat-tolerant grape cultivars through genetic manipulation of HSFB1.
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