Complete Genome Sequence of Staphylococcus epidermidis CCSM0287, Isolated from Healthy Facial Skin

Area of Science:

• Microbiology
• Genomics
• Bioinformatics

Background:

• Staphylococcus epidermidis is a common skin commensal.
• Understanding its genomic diversity is crucial for microbiome research.

Purpose of the Study:

• To present the complete genome sequence of Staphylococcus epidermidis strain CCSM0287.
• To characterize the genomic structure, including chromosomal and plasmid content.

Main Methods:

• Isolation of Staphylococcus epidermidis strain CCSM0287 from healthy human facial skin.
• Whole-genome sequencing using Pacific Biosciences (PacBio) RS II single-molecule real-time (SMRT) and Illumina sequencing technologies.
• Bioinformatic assembly and analysis of the sequenced genome.

Main Results:

• The complete genome size of strain CCSM0287 was determined to be 2.5 Mbp.
• The genome comprises a single chromosome and three distinct plasmids.
• High-quality genome assembly was achieved through hybrid sequencing approaches.

Conclusions:

• The genome sequence provides a valuable resource for studying Staphylococcus epidermidis.
• Characterization of plasmids offers insights into mobile genetic elements and adaptation.
• This work contributes to the genomic understanding of skin commensal bacteria.