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Urinary BLACAT1 as a non-invasive biomarker for bladder cancer
Fathia Z El Sharkawi1, Mahmoud El Sabah2, Hanaa B Atya3
1Biochemistry and Molecular Biology Department, Faculty of Pharmacy, Helwan University, P.O. Box 11795, Cairo, Egypt.
Molecular Biology Reports
|March 20, 2023
Summary
Urinary long non-coding RNA BLACAT1 shows promise as a biomarker for bladder cancer (BC). Its levels indicate disease stage and metastasis risk, offering a non-invasive diagnostic tool.
Area of Science:
- Oncology
- Molecular Biology
- Biomarker Discovery
Background:
- Bladder cancer (BC) has high morbidity and mortality, with frequent recurrence from non-muscle-invasive to muscle-invasive stages.
- Limited diagnostic biomarkers exist for BC, necessitating novel markers for early detection and prognosis.
- Urinary long non-coding RNA BLACAT1 is investigated for its potential as a non-invasive biomarker.
Purpose of the Study:
- To determine the expression and clinical significance of urinary BLACAT1 in bladder cancer patients.
- To evaluate BLACAT1 as a non-invasive diagnostic and prognostic biomarker for BC.
- To differentiate between various stages of BC, including metastatic potential.
Main Methods:
- Quantitative real-time PCR (qRT-PCR) was used to measure urinary BLACAT1 levels.
- Expression levels were analyzed in 70 BC patients across different TNM stages (T0-T3) and 12 healthy controls.
- Correlation of BLACAT1 levels with disease progression and metastasis was assessed.
Main Results:
- BLACAT1 was downregulated in superficial BC stages (T0, T1) compared to controls.
- BLACAT1 levels significantly elevated in invasive stages (T2, T3), correlating positively with disease progression.
- Urinary BLACAT1 effectively differentiated between metastatic and non-metastatic BC stages.
Conclusions:
- Upregulation of BLACAT1 in invasive BC stages indicates an unfavorable prognosis.
- BLACAT1 contributes to BC cell migration and metastasis.
- Urinary BLACAT1 is a promising non-invasive biomarker for detecting BC metastasis.
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