Anthocyanin-induced color changes in bacterial cellulose nanofibers for the accurate and selective detection of...

The environment and our health are negatively impacted by heavy metal ions, like Cu(II). The present study developed a green and effective metallochromic sensor that detects copper (Cu(II)) ions in solution and solid state using anthocyanin extract from black eggplant peels embedded in bacterial cellulose nanofibers (BCNF). Cu(II) is quantitatively detected by the sensing method with detection limits between 10-400 ppm and 20-300 ppm in solution and solid state, respectively. In the solution state, we depicted a sensor for Cu(II) ions in aqueous matrices in the pH range from 3.0 to 11.0, with the capability to produce a visual color change from brown to light blue and dark blue depending on the Cu(II) concentration. Additionally, BCNF-ANT film can act as a sensor for Cu(II) ions in the pH range of 4.0-8.0. Neutral pH was selected from the standpoint of high selectivity. It was found that visible color changed when Cu(II) concentration was increased. Bacterial cellulose nanofibers modified with anthocyanin were characterized with ATR-FTIR and FESEM. Various metal ions, including Pb²⁺, Co²⁺, Zn²⁺, Ni²⁺, Al³⁺, Ba²⁺, Hg²⁺, Mg²⁺, and Na⁺, were used to challenge the sensor to determine its selectivity. Anthocyanin solution and BCNF-ANT sheet were employed in the actual tap water sample successfully. The results also clarified that the various foreign ions did not significantly interfere with Cu(II) ions detection at optimum conditions. Compared to previously developed sensors, no electronic components, trained personnel, or sophisticated equipment were needed to apply the colorimetric sensor developed in this research. Cu(II) contamination in food matrices and water can be monitored on-site easily.