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[Democratization of Education: Adaptive Learning Courses in Laboratory Medicine Are Now Available without A

Nader Rifai1

  • 1Past Editor-In-Chief, Clinical Chemistry, Co-Editor, AACC Learning Lab, Boston Children's Hospital Department of Laboratory Medicine, 300 Longwood Avenue Boston, MA 02115 USA.

Annales De Biologie Clinique
|March 20, 2023
PubMed
Abstract

No abstract available in PubMed .

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.