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Correlations between intravoxel incoherent motion-derived fast diffusion and perfusion fraction parameters and VEGF-
Yaru Sheng1, Xuefei Dang2, Hua Zhang3
1Radiology Department of Huashan Hospital, Fudan University, Shanghai, 200040, China.
Objectives:
To explore the correlations between histopathologic findings and intravoxel incoherent motion (IVIM)-derived perfusion and diffusion parameters in brain gliomas.
Methods:
Thirty-two biopsy samples from twenty-one patients with newly diagnosed gliomas from a previous prospective cohort study were retrospectively analyzed. All patients underwent diffusion-weighted MRI with 22 b values (0-5000 s/mm2), followed by intraoperative MR-guided biopsy surgery and surgical resection. All 32 biopsy samples underwent immunohistochemical staining followed by quantitative analysis of cell density (cellularity), percent of MIB-1 (Ki67)-positive expression (pMIB-1), number of CD34-stained vessels (CD34-MVD), and percent of VEGF-positive expressing cells (pVEGF) using a multispectral phenotyping microscope. Based on the co-registered localized biopsy, correlation analysis was performed between the IVIM-derived biexponential model-based parameters (Dfast1500 and Dfast5000, Dslow1500 and Dslow5000, PF1500 and PF5000) and the above four pathological biomarkers and glioma grades.
Results:
Significant positive correlations were revealed between Dfast5000 and pVEGF (rho (r) = 0.466, p = 0.007), and Dfast1500 and pVEGF (r = 0.371, p = 0.037). A significant negative correlation was revealed between PF5000 with pMIB-1 (r = - 0.456, p = 0.01). Moderate to good positive correlations were shown between Dfast5000 and glioma grades (r = 0.509, p = 0.003) and Dfast1500 and glioma grades (r = 0.476, p = 0.006).
Conclusions:
IVIM-DWI-derived Dfast and PF correlate, respectively, with intratumor pVEGF and pMIB-1. When using the wide-high b value scheme, IVIM-derived Dfast and PF tend to demonstrate better efficacy in evaluating malignancy-related characteristics such as angiogenesis and cellular proliferation in gliomas.
Key Points:
• Intravoxel incoherent motion-diffusion-weighted imaging (IVIM-DWI)-derived fast diffusion (Dfast) and perfusion fraction (PF) can quantitatively reflect intratumor pVEGF and pMIB-1. • IVIM-DWI-derived Dfast and PF tend to demonstrate better efficacy in evaluating glioma malignancy when an optimized scheme is used. • IVIM-DWI-derived Dfast5000 and PF5000 are promising non-invasive parameters correlating with pVEGF and pMIB-1 in gliomas.
Insights
Intravoxel incoherent motion-diffusion-weighted imaging (IVIM-DWI) fast diffusion (Dfast) and perfusion fraction (PF) correlate with glioma malignancy markers. Optimized IVIM-DWI parameters show promise for non-invasively assessing tumor characteristics like angiogenesis and proliferation.
Area of Science:
- Radiology and Imaging Science
- Neuro-oncology
- Biomedical Engineering
Background:
- Brain gliomas are complex tumors requiring accurate assessment of malignancy.
- Histopathologic findings are crucial but invasive; non-invasive imaging biomarkers are needed.
- Intravoxel incoherent motion (IVIM)-diffusion-weighted imaging (DWI) offers insights into tissue microstructures.
Purpose of the Study:
- To investigate correlations between histopathologic findings and IVIM-derived parameters in brain gliomas.
- To assess the potential of IVIM-DWI parameters to reflect tumor characteristics such as vascularity and proliferation.
Main Methods:
- Retrospective analysis of 32 biopsy samples from 21 glioma patients.
- Diffusion-weighted MRI with 22 b values (0-5000 s/mm²) followed by biopsy.
- Immunohistochemical analysis for cell density, MIB-1 (Ki67), CD34-MVD, and VEGF; correlation with IVIM parameters (Dfast, Dslow, PF).
Main Results:
- Significant positive correlations found between Dfast (at 1500 and 5000 s/mm²) and percent VEGF (pVEGF).
- Significant negative correlation observed between PF (at 5000 s/mm²) and percent MIB-1 (pMIB-1).
- Moderate to good positive correlations noted between Dfast (at 1500 and 5000 s/mm²) and glioma grades.
Conclusions:
- IVIM-DWI-derived Dfast and PF quantitatively correlate with intratumor pVEGF and pMIB-1, respectively.
- Optimized IVIM-DWI schemes, particularly with higher b values, enhance evaluation of glioma malignancy.
- IVIM-derived Dfast5000 and PF5000 show promise as non-invasive biomarkers for assessing glioma angiogenesis and proliferation.
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