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CircRANBP17 modulated KDM1A to regulate neuroblastoma progression by sponging miR-27b-3p

Lijun Zhao1, Junying Fan2, Chunyang Zhang3

  • 1Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, Suzhou City, Jiangsu Province, 215000, China.

Open Medicine (Warsaw, Poland)
|March 21, 2023
PubMed

Insights

Circular RNA RAN binding protein 17 (circRANBP17) promotes neuroblastoma (NB) progression by sponging miR-27b-3p and upregulating KDM1A. Knockdown of circRANBP17 inhibits NB tumor formation, offering a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Neuroblastoma (NB) is a prevalent pediatric cancer.
  • Circular RNAs (circRNAs) are increasingly recognized for their roles in tumor progression.
  • circRANBP17 has been implicated in various cancers, but its specific role in NB requires elucidation.

Purpose of the Study:

  • To investigate the function and underlying mechanism of circRANBP17 in neuroblastoma.
  • To explore the regulatory network involving circRANBP17, miR-27b-3p, and KDM1A in NB.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) to measure expression levels of circRANBP17, miR-27b-3p, and KDM1A.
  • In vivo mouse models to assess the impact of circRANBP17 knockdown on tumor formation.
  • Functional assays to evaluate cell proliferation, migration, invasion, and apoptosis.

Main Results:

  • circRANBP17 and KDM1A were significantly upregulated, while miR-27b-3p was downregulated in NB tissues and cells.
  • circRANBP17 knockdown inhibited NB cell proliferation, migration, and invasion, and promoted apoptosis.
  • miR-27b-3p targeted KDM1A, and its inhibition reversed the effects of circRANBP17 knockdown on NB cells.
  • circRANBP17 knockdown suppressed tumor formation in vivo.

Conclusions:

  • circRANBP17 promotes NB progression by sponging miR-27b-3p, leading to KDM1A upregulation.
  • This regulatory axis (circRANBP17/miR-27b-3p/KDM1A) plays a critical role in NB cell malignancy.
  • The findings provide a potential theoretical basis for novel therapeutic strategies against neuroblastoma.
Keywords:
KDM1AcircRANBP17miR-27b-3pneuroblastoma

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