Related Experiment Video
Updated: Aug 6, 2025
Development of Compendium for Esophageal Squamous Cell Carcinoma
Published on: April 12, 2024
Long noncoding RNA LINC01088 inhibits esophageal squamous cell carcinoma progression by targeting the NPM1-HDM2-p53
Fan Liang1, Qiuli Luo2, Haibo Han3
1Department of Thoracic Surgery II, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Peking University Cancer Hospital & Institute, Beijing 100142, China.
Abstract:
Esophageal squamous cell carcinoma (ESCC) is characterized by extensive metastasis and poor prognosis. Long noncoding RNAs (lncRNAs) have been shown to play important roles in ESCC. However, the specific roles of lncRNAs in ESCC tumorigenesis and metastasis remain largely unknown. Here, we investigate LINC01088 in ESCC. Differentially expressed LINC01088 levels are screened from the GEO database. We find that LINC01088 is expressed at low level in collected clinical samples and is correlated with vascular tumor emboli and poor overall survival time of patients after surgery. LINC01088 inhibits not only ESCC cell migration and invasion in vitro, but also tumorigenesis and metastasis in vivo. Mechanistically, LINC01088 directly interacts with nucleophosmin (NPM1) and increases the expression of NPM1 in the nucleoplasm compared to that in the nucleolar region. LINC01088 decreases mutant p53 (mut-p53) expression and rescues the transcriptional activity of p53 by targeting the NPM1-HDM2-p53 axis. LINC01088 may also interfere with the DNA repair function of NPM1 by affecting its translocation. Our results highlight the potential of LINC01088 as a prognostic biomarker and therapeutic target of ESCC.
Insights
Low levels of LINC01088 long noncoding RNA (lncRNA) are linked to poor outcomes in esophageal squamous cell carcinoma (ESCC). This study shows LINC01088 inhibits ESCC progression and metastasis.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Esophageal squamous cell carcinoma (ESCC) presents significant metastatic potential and poor patient prognosis.
- Long noncoding RNAs (lncRNAs) are implicated in ESCC, but their specific roles in tumorigenesis and metastasis require further elucidation.
Purpose of the Study:
- To investigate the role of LINC01088 in the development and metastasis of ESCC.
- To explore LINC01088 as a potential prognostic biomarker and therapeutic target for ESCC.
Main Methods:
- Differential expression analysis of LINC01088 using the GEO database.
- In vitro assays (cell migration and invasion) and in vivo studies to assess LINC01088 function.
- Mechanistic studies involving nucleophosmin (NPM1), mutant p53 (mut-p53), and the NPM1-HDM2-p53 axis.
Main Results:
- LINC01088 was found to be downregulated in clinical ESCC samples and correlated with vascular invasion and reduced overall survival.
- LINC01088 suppressed ESCC cell migration, invasion, tumorigenesis, and metastasis both in vitro and in vivo.
- LINC01088 interacts with NPM1, affecting its nucleoplasmic expression and targeting the NPM1-HDM2-p53 pathway to decrease mut-p53 and restore p53 activity.
Conclusions:
- LINC01088 functions as a tumor suppressor in ESCC by inhibiting metastasis and tumorigenesis.
- LINC01088 holds promise as a prognostic biomarker and a potential therapeutic target for esophageal squamous cell carcinoma.
Related Concept Videos
02:23Abnormal Proliferation
02:57Targeted Cancer Therapies
There are several types of targeted therapies against...
02:07Tumor Progression
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...