Smart Lattice Light Sheet Microscopy for imaging rare and complex cellular events

Area of Science:

• Cell Biology
• Microscopy Technology

Background:

• Biological processes occur across diverse spatiotemporal scales.
• Capturing rare or transient events requires adaptable imaging techniques.

Approach:

• Developed smartLLSM, an AI-controlled microscope switching between epifluorescent and lattice light sheet microscopy.
• Implemented real-time image feedback for autonomous mode switching.

Key Points:

• Achieved population-level cell cycle statistics across thousands of cells.
• Autonomously captured multicolor 3D datasets and 4D time-lapse movies of cell division.
• Enabled quantitative image analysis of kinetochore and chromosome dynamics.

Conclusions:

• Efficiently detects rare events in heterogeneous cell populations.
• Facilitates high spatiotemporal 4D imaging for statistically significant replicates.
• Reveals drug perturbation effects on specific mitotic stages.