Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitotic Cdc42 waves encode PI(3,4)P<sub>2</sub> signaling and Golgi morphological state to control spindle scaling.

Science advances·2026
Same author

PD-1 signaling and PD-1 blockade-mediated tumor control are established at microvillar T cell contacts.

Science immunology·2026
Same author

Implementation of an adaptive-optics assisted isoSTED nanoscope.

Nature protocols·2026
Same author

Fluorogenic speed-optimized DNA-PAINT probes enable super-resolution imaging of whole cells.

bioRxiv : the preprint server for biology·2026
Same author

Evaluating MINFLUX experimental performance in silico.

Nature communications·2025
Same author

All-optical visualization of specific molecules in the ultrastructural context of brain tissue.

Nature biotechnology·2025
Same journal

A human-specific genetic modifier reconfigures large-scale cortical network dynamics underlying behavioral performance.

bioRxiv : the preprint server for biology·2026
Same journal

<i>Staphylococcus aureus</i> uses a eukaryotic-like uridyltransferase to make UDP-GlcNAc for cell wall synthesis.

bioRxiv : the preprint server for biology·2026
Same journal

Dynamic redistribution of eIF4F controls cap-dependent translation initiation.

bioRxiv : the preprint server for biology·2026
Same journal

When does additional information improve accuracy of RNA secondary structure prediction?

bioRxiv : the preprint server for biology·2026
Same journal

Normative brain-state trajectories reveal deviation from healthy aging in Alzheimer's disease.

bioRxiv : the preprint server for biology·2026
Same journal

Noradrenergic infraslow rhythm during sleep is the critical link between heart-rate dynamics and memory consolidation.

bioRxiv : the preprint server for biology·2026
See all related articles

Related Experiment Video

Updated: Aug 6, 2025

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

8.5K

Extracting nanoscale membrane morphology from single-molecule localizations.

Zach Marin, Lukas A Fuentes, Joerg Bewersdorf

    Biorxiv : the Preprint Server for Biology
    |March 22, 2023
    PubMed
    Summary
    This summary is machine-generated.

    NanoWrap enables nanometer-scale membrane surface reconstruction from single-molecule localization microscopy (SMLM) data. This method allows detailed study of subcellular structures, overcoming limitations of previous techniques.

    More Related Videos

    Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy
    13:13

    Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy

    Published on: December 3, 2012

    16.2K
    Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy
    09:09

    Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy

    Published on: March 5, 2021

    4.4K

    Related Experiment Videos

    Last Updated: Aug 6, 2025

    Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
    11:06

    Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

    Published on: June 30, 2018

    8.5K
    Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy
    13:13

    Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy

    Published on: December 3, 2012

    16.2K
    Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy
    09:09

    Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy

    Published on: March 5, 2021

    4.4K

    Area of Science:

    • Cellular Biology
    • Biophysics
    • Microscopy

    Background:

    • Nanometer-scale membrane surface reconstruction is crucial for understanding subcellular dynamics.
    • Electron microscopy faces challenges in extracting surfaces from specific labels.
    • Current fluorescence microscopy methods offer limited resolution or require extensive manual data curation.

    Approach:

    • Introduced NanoWrap, a novel computational method for surface extraction from generalized single-molecule localization microscopy (SMLM) data.
    • Developed an open-source implementation within the Python Microscopy Environment.
    • Validated NanoWrap through simulations and application to real SMLM datasets.

    Key Points:

    • NanoWrap achieves high-resolution membrane surface reconstruction from SMLM data.
    • Enables the study of specifically-labeled membranous structures within cells.
    • Overcomes resolution and data quality limitations of existing fluorescence microscopy techniques.

    Conclusions:

    • NanoWrap significantly advances the capability to analyze subcellular structure and dynamics.
    • Provides a robust tool for researchers studying cell morphology and membrane-bound organelles.
    • Facilitates detailed investigations into the mechanisms of subcellular shape change.