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Author Spotlight: Universal Molecular Retention with 11-Fold Expansion Microscopy
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Author Spotlight: Universal Molecular Retention with 11-Fold Expansion Microscopy

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MicroMagnify: a multiplexed expansion microscopy method for pathogens and infected tissues

Yongxin Zhao1, Zhangyu Cheng1, Caroline Stefani2

  • 1Carnegie Mellon University.

Research Square
|March 22, 2023
PubMed

Insights

We developed µMagnify, a novel nanoscale imaging method for visualizing pathogens and infected tissues. This technique enhances super-resolution microscopy for better understanding microbial-host interactions and developing new diagnostics.

Area of Science:

  • Microbiology
  • Microscopy
  • Pathogen research

Background:

  • Super-resolution optical imaging is vital for microbiology but limited for pathogens and infected tissues.
  • Understanding microbial structures and host interactions requires advanced imaging techniques.

Approach:

  • Developed µMagnify, a nanoscale multiplexed imaging method using expansion microscopy and a universal biomolecular anchor.
  • Formulated an enzyme cocktail for robust microbial cell wall digestion and expansion, preserving biomolecules for high-plex fluorescence imaging.
  • Created a virtual reality tool for immersive 3D image visualization and collaborative exploration.

Key Points:

  • µMagnify achieves nanoscale precision in imaging microbial pathogens and infected tissues.
  • The method ensures robust cell expansion without biomolecular distortion.
  • Enables high-plex fluorescence imaging for detailed analysis.

Conclusions:

  • µMagnify is a powerful imaging platform for studying microbe-host interactions.
  • This technology can advance the development of diagnostic strategies for infectious diseases.

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