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Transcription Factor Dynamics in Cross-Regulation of Plant Hormone Signaling Pathways
Lingling Yin1,2, Mark Zander3,4,5,6, Shao-Shan Carol Huang3,4,5,7
1La Trobe Institute for Agriculture and Food, Department of Animal, Plant and Soil Sciences, School of Agriculture Biomedicine and Environment, AgriBio Building, La Trobe University, Melbourne, VIC 3086, Australia.
Plant hormones coordinate growth through complex cross-regulatory networks. This study reveals how transcription factors and MITOGEN-ACTIVATED PROTEIN KINASES (MPKs) integrate signals and influence gene expression and alternative splicing.
Area of Science:
- Plant Biology
- Molecular Plant Science
- Systems Biology
Background:
- Plant growth and development rely on intricate hormone signaling pathways.
- Understanding the molecular basis of cross-regulation among multiple hormones is crucial.
Approach:
- Generated a cross-regulation network for six key plant hormones in etiolated Arabidopsis seedlings.
- Reconstructed dynamic transcriptional regulatory models incorporating transcription factor activity and protein-protein interactions.
- Investigated the role of MITOGEN-ACTIVATED PROTEIN KINASES (MPKs) in hormone cross-regulation.
Key Points:
- Hormone signaling involves distinct yet overlapping sets of transcription factors.
- Hub target genes and MPKs act as critical nodes in hormone cross-regulation.
- Hormones induce significant alternative splicing with distinct transcriptomic effects.
Conclusions:
- This research provides a comprehensive view of plant transcriptional regulatory pathways and hormone cross-talk.
- Identified MPK6 as a key regulator whose loss impacts proteome, phosphoproteome, and transcriptome.
- Demonstrated that alternative splicing is an early and significant response to hormonal stimuli.
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