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Updated: Aug 6, 2025

Generation of Orthotopic Pancreatic Tumors and Ex vivo Characterization of Tumor-Infiltrating T Cell Cytotoxicity
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Multiomics Empowers Predictive Pancreatic Cancer Immunotherapy

Janelle M Montagne1,2,3, Elizabeth M Jaffee1,2,3, Elana J Fertig1,2

  • 1Department of Oncology, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD.

Journal of Immunology (Baltimore, Md. : 1950)
|March 22, 2023
PubMed

Insights

Pancreatic cancer (PDAC) resists immunotherapy due to its complex tumor microenvironment. New multiomics and single-cell analyses can identify biomarkers to improve treatment responses.

Area of Science:

  • Oncology and Immunology
  • Cancer Research
  • Translational Medicine

Background:

  • Cancer immunotherapy, especially immune checkpoint inhibitors, has transformed outcomes for many advanced cancers.
  • Pancreatic ductal adenocarcinoma (PDAC) patients typically exhibit poor responses to current immunotherapies.
  • PDAC's resistance is attributed to late diagnosis, low mutation rates, and an immunosuppressive tumor microenvironment.

Approach:

  • Interrogating the PDAC tumor microenvironment requires simultaneous analysis of cancer and immune cellular components.
  • Single-cell and multiomics technologies offer powerful tools for dissecting complex cellular interactions.
  • Advanced analytical methods are essential for interpreting the vast datasets generated by these technologies.

Key Points:

  • Identifying biomarkers for immunotherapeutic resistance and response is critical for PDAC treatment.
  • Multiomics and single-cell analyses enable systems-level understanding of PDAC's immune evasion mechanisms.
  • These approaches facilitate the discovery of novel therapeutic targets and strategies.

Conclusions:

  • Multiomics and single-cell analyses provide unprecedented opportunities to understand PDAC immunotherapy resistance.
  • These advanced techniques can identify biomarkers to sensitize PDAC to immunotherapy.
  • This research paves the way for developing more effective treatments for pancreatic cancer.

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