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Updated: Aug 6, 2025
10:03
Generation of Induced Pluripotent Stem Cells from Human Melanoma Tumor-infiltrating Lymphocytes
Published on: November 11, 2016
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Apoptosis inhibition enhances induced pluripotent stem cell generation during T cell reprogramming
Jiangtao Ren1, Xuhua Zhang2, Zhenhui Zhang3
1Department of Oncology, State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, National Clinical Research Center for Respiratory Disease, Guangzhou Medical University, Guangzhou, 510530, China; Nanjing Bioheng Biotech Co., Ltd, Nanjing, Jiangsu, China.
Biochemical and Biophysical Research Communications
|March 22, 2023
Summary
Generating induced pluripotent stem cells (iPSCs) for immunotherapy is challenging. Blocking Fas-FasL apoptosis in T cells improves iPSC generation, offering a new method for high-quality cellular immunotherapy.
Area of Science:
- Cellular immunotherapy
- Stem cell biology
- Immunological engineering
Background:
- Chimeric antigen receptor (CAR)-T cell therapy faces manufacturing challenges.
- Induced pluripotent stem cells (iPSCs) offer a promising alternative for immunotherapy due to self-renewal and differentiation capabilities.
- The epigenetic imprint of somatic cells can affect iPSC differentiation, necessitating careful cell selection and reprogramming efficiency for immunotherapy.
Purpose of the Study:
- To investigate the superiority of lymphocyte-origin cells over embryonic stem cells for functional immune cell differentiation.
- To explore methods for improving induced pluripotent stem cell (iPSC) generation efficiency for immunotherapy.
- To assess the impact of transient Fas suppression on T cell reprogramming and subsequent iPSC differentiation.
Main Methods:
- Comparative analysis of immune cell differentiation from lymphocyte-origin iPSCs versus embryonic stem cells.
- Experimental blocking of Fas-FasL induced apoptosis in T cells during the reprogramming process.
- Assessment of Fas expression and differentiation potential in iPSCs derived from transiently Fas-suppressed T cells.
Main Results:
- Lymphocyte-origin cells demonstrated superior functional immune cell differentiation compared to embryonic stem cells.
- Blocking Fas-FasL induced apoptosis in T cells significantly enhanced iPSC generation efficiency.
- Transient Fas suppression in T cells did not affect Fas expression in the resulting iPSCs or their differentiation capacity.
Conclusions:
- Lymphocyte-derived iPSCs are superior for generating functional immune cells for immunotherapy.
- Transient Fas suppression is a viable strategy to improve iPSC generation from T cells without compromising their differentiation potential.
- This research provides a novel approach for producing high-quality iPSCs for advanced cellular immunotherapy applications.
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