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Related Experiment Video

Updated: Aug 6, 2025

Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives

Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives

Published on: September 16, 2021

Is isochoric vitrification feasible?

Prem K Solanki¹, Yoed Rabin¹

¹Biothermal Technology Laboratory, Department of Mechanical Engineering, Carnegie Mellon University, Pittsburgh, PA, 15213, USA.

|March 22, 2023

Summary

This summary is machine-generated.

Ice-free isochoric vitrification is not feasible for cryopreservation. Mathematical modeling shows CPA solution contraction prevents necessary pressure buildup in isochoric chambers, hindering amorphous solid formation.

Keywords:

Computation Isochoric cryopreservation Mathematical modeling Vitrification

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Area of Science:

Cryopreservation
Biophysics
Materials Science

Background:

Isochoric cryopreservation utilizes sealed containers to elevate pressure via ice formation for biological preservation.
Vitrification involves rapid cooling to form an amorphous solid without ice crystals.
Recent interest in isochoric vitrification follows positive experimental outcomes in isochoric cryopreservation.

Purpose of the Study:

To investigate the feasibility of ice-free isochoric vitrification for cryopreservation.
To develop a mathematical model for vitrification under variable pressure conditions.
To assess the role of thermo-mechanics in isochoric vitrification.

Main Methods:

Mathematical modeling and computation tools.
Application of thermo-mechanics principles.
Simulation using physical properties of dimethyl sulfoxide (DMSO) as a cryoprotective agent (CPA).

Main Results:

Vitrification under isochoric conditions was found to be unfeasible.
Cryoprotective agent solutions contract significantly more than the isochoric chamber.
This differential contraction can lead to negative pressures, negating the process's premise.

Conclusions:

Achieving vitrification in isochoric chambers is not feasible due to differential contraction.
External pressure application is necessary to prevent ice formation and leverage high-pressure benefits.
Passive isochoric chambers are insufficient for successful isochoric vitrification.