Atractylodes macrocephala Rhizomes Contain Anti-inflammatory Sesquiterpenes
Cao Thanh Hai1,2,3, Nguyen Thi Luyen2, Do Hoang Giang2
1Graduate University of Science and Technology, Vietnam Academy of Science and Technology (VAST).
Chemical & Pharmaceutical Bulletin
|March 22, 2023
Summary
Researchers isolated five compounds from Atractylodes macrocephala rhizomes. Atractylenolide III (5) demonstrated significant anti-inflammatory effects by inhibiting nitric oxide production, while other compounds showed moderate to no activity.
Area of Science:
- Phytochemistry
- Natural Products Chemistry
- Pharmacology
Background:
- Atractylodes macrocephala is a traditional medicinal herb.
- Sesquiterpene lactones are a class of natural products with diverse biological activities.
Purpose of the Study:
- To isolate and characterize compounds from Atractylodes macrocephala rhizomes.
- To evaluate the anti-inflammatory potential of the isolated compounds.
Main Methods:
- Isolation of compounds using chromatographic techniques.
- Structure elucidation using 1D and 2D-NMR spectroscopy and HR-ESI-MS.
- In vitro anti-inflammatory assay measuring nitric oxide production inhibition.
Main Results:
- Two new compounds, eudesm-4(15),7-diene-3α,9β,11-triol (1) and eudesm-4(15),7-diene-1β,3α,9β,11-tetraol (2), were identified.
- Three known sesquiterpene lactones, (1S,5R,7R,10R)-secoatractylolactone (3), (1S,5R,7R,10R)-secoatractylolactone-11-O-β-D-glucopyranoside (4), and atractylenolide III (5), were also isolated.
- Atractylenolide III (5) showed the most potent anti-inflammatory activity with an IC50 of 27.5 µM against nitric oxide production.
- Compounds 1, 2, and 3 exhibited moderate anti-inflammatory effects, while compound 4 was inactive.
Conclusions:
- Atractylodes macrocephala rhizomes are a source of bioactive sesquiterpene lactones.
- Atractylenolide III is a promising anti-inflammatory agent.
- Further research into the anti-inflammatory mechanisms of these compounds is warranted.
