Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Association between an inpatient oral self-care promotion programme and hospital-acquired infections: a propensity score-matched cohort study.

The Journal of hospital infection·2026
Same author

Prevalence and clinical significance of germline pathogenic variants identified by multigene panel testing in high-risk Japanese patients with breast cancer.

Breast cancer (Tokyo, Japan)·2026
Same author

A Knock-In Igfn1<sup>iCre</sup> transgenic mouse line provides partial developmental access to type-7 bipolar cells.

Neuroscience research·2026
Same author

Mechanistic insights into dentin bridge properties in a vital pulp therapy mimetic murine model.

Journal of the mechanical behavior of biomedical materials·2026
Same author

iGABASnFR2 is an improved genetically encoded protein sensor of GABA.

eLife·2026
Same author

Risk factors for postextubation pneumonia using diagnosis procedure combination and claims data in Japan.

Scientific reports·2026

Related Experiment Video

Updated: Aug 6, 2025

Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier
10:16

Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier

Published on: February 8, 2017

7.6K

Targeting cellular gaps using Janus nanoparticles containing cationic polymers and surfactant lipids.

Akihiro Matsumoto1, Takeo Kitazawa2, Yuta Hatori2

  • 1Laboratory of Pharmaceutics, Faculty of Pharmacy, Osaka Ohtani University, Osaka, Japan.

Drug Discoveries & Therapeutics
|March 22, 2023
PubMed
Summary

Janus nanoparticles, unlike spherical ones, show specific localization near cell junctions in colon cancer cells. This finding highlights their potential as targeted intracellular drug carriers for cellular gaps.

Keywords:
adherens junctioncationic polymercellular uptakehuman Caucasian colon adenocarcinoma (Caco-2) cellsurfactant lipids

More Related Videos

Solid Lipid Nanoparticles SLNs for Intracellular Targeting Applications
08:19

Solid Lipid Nanoparticles SLNs for Intracellular Targeting Applications

Published on: November 17, 2015

17.9K
A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles
09:57

A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles

Published on: December 23, 2016

8.9K

Related Experiment Videos

Last Updated: Aug 6, 2025

Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier
10:16

Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier

Published on: February 8, 2017

7.6K
Solid Lipid Nanoparticles SLNs for Intracellular Targeting Applications
08:19

Solid Lipid Nanoparticles SLNs for Intracellular Targeting Applications

Published on: November 17, 2015

17.9K
A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles
09:57

A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles

Published on: December 23, 2016

8.9K

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Cell Biology

Background:

  • Nanoparticles are investigated as intracellular drug carriers due to cellular uptake mechanisms.
  • Janus particles, with their distinct domains, offer unique properties for applications like imaging and sensing.

Purpose of the Study:

  • To investigate how nanoparticle type influences distribution within a human colon adenocarcinoma (Caco-2) cell monolayer.
  • To evaluate the potential of Janus nanoparticles as targeted drug delivery systems.

Main Methods:

  • Fabrication of Janus and conventional spherical nanoparticles using pharmaceutical ingredients (cationic polymer and surfactant lipids).
  • Preparation via solvent evaporation and diffusion methods, controlling solvent removal.
  • Evaluation of nanoparticle distribution in Caco-2 cells using confocal laser microscopy.

Main Results:

  • Janus nanoparticles exhibited a mean hydrodynamic size of 119.2 ± 4.6 nm.
  • Janus nanoparticles localized specifically around adherens junctions below tight junctions in Caco-2 cells.
  • Non-Janus nanoparticles did not show similar specific localization.

Conclusions:

  • The asymmetric structure and positive charge of Janus nanoparticles likely contribute to their targeted localization.
  • Janus nanoparticles show significant potential for developing drug carriers that target cellular gaps.