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RNF150 suppresses papillary thyroid carcinoma with ASK1 ubiquitination presenting a direct target via inactivating

Wei Deng1,2, Jing Wu2, Wei Zheng3

  • 1Department of Endocrinology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.

Cell Biology International
|March 23, 2023
PubMed

Insights

Ring finger protein 150 (RNF150) suppresses papillary thyroid carcinoma (PTC) growth by inhibiting the p38 pathway and promoting ASK1 ubiquitination. High RNF150 expression correlates with better prognosis in PTC patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Papillary thyroid carcinoma (PTC) is the most common endocrine malignancy, but its recurrence mechanisms are not fully understood.
  • The role of the RING finger protein (RNF) family, specifically RNF150, in PTC pathogenesis requires further investigation.

Purpose of the Study:

  • To investigate the function and molecular mechanism of RNF150 in papillary thyroid carcinoma.
  • To analyze the expression and prognostic value of RNF150 in thyroid cancer using The Cancer Genome Atlas-THCA (TCGA-THCA) dataset.

Main Methods:

  • Analysis of TCGA-THCA dataset for RNF150 expression and correlation with patient prognosis.
  • In vitro and in vivo experiments to assess the effect of RNF150 on PTC cell proliferation and p38 phosphorylation.
  • Investigation of RNF150 interaction with ASK1 and its ubiquitination status.
  • Assessment of p38 pathway activity using a p38 inhibitor.

Main Results:

  • RNF150 was found to be lowly expressed in THCA, with high expression indicating a favorable prognosis.
  • RNF150 inhibited PTC cell proliferation and suppressed p38 phosphorylation both in vitro and in vivo.
  • Knockdown of RNF150 promoted PTC cell proliferation and p38 phosphorylation, effects reversible by a p38 inhibitor.
  • RNF150 interacted with ASK1, promoting its ubiquitination, and RNF150 and ASK1 showed reversed expression patterns in PTC specimens.

Conclusions:

  • RNF150 suppresses PTC proliferation by inactivating the p38 pathway and promoting ASK1 ubiquitination.
  • RNF150 represents a potential therapeutic target for papillary thyroid carcinoma treatment.
Keywords:
RNF150cell cycleproliferationubiquitination

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