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Rh single-atom nanozymes for efficient ascorbic acid oxidation and detection.

Xiaoyue Shi1,2, Juan Li1, Yu Xiong3

  • 1School of Chemistry and Chemical Engineering, Shandong University, 250100 Jinan, China. bin.cai@sdu.edu.cn.

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Summary

We developed Rh single-atom nanozymes (Rh SAzymes) that mimic enzymes for efficient ascorbic acid (AA) detection. This breakthrough enables a new biosensor for tracking AA in humans and products.

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Area of Science:

  • Electrochemistry
  • Nanomaterials Science
  • Biomedical Sensing

Background:

  • Ascorbic acid (AA) management is crucial for health but detecting it in biological fluids is challenging.
  • Existing sensing catalysts lack high performance for accurate AA detection.

Purpose of the Study:

  • To design Rh single-atom nanozymes (Rh SAzymes) that mimic ascorbate peroxidase active sites.
  • To develop a high-performance electrocatalyst for ascorbic acid (AA) oxidation and detection.
  • To construct a miniaturized biosensor for real-time AA monitoring.

Main Methods:

  • Synthesized Rh single-atom nanozymes (Rh SAzymes) by mimicking enzyme active sites.
  • Utilized screen-printing technology to fabricate a miniaturized biosensor.
  • Evaluated electrocatalytic activity and detection performance of the Rh SAzyme biosensor.

Main Results:

  • Rh SAzymes demonstrated unprecedented electrocatalytic activity for AA oxidation with a low onset potential (0.02 V vs. Ag/AgCl).
  • The biosensor achieved a wide linear detection range (10.0 μM-53.1 mM) and a low detection limit (0.26 μM).
  • The biosensor exhibited excellent stability over 28 days and enabled dynamic AA trend tracking in human subjects.

Conclusions:

  • Rh SAzymes offer a novel approach for efficient AA electrocatalytic oxidation and detection.
  • The developed biosensor provides a sensitive, stable, and practical platform for AA monitoring.
  • This work paves the way for designing single-atom electrocatalysts for advanced biosensing applications.