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Detection of Peroxisomal Proteins During Mycobacterial Infection
Ananyaashree Behera1, Mainak Biswas1, Süleyman Ergün2
1School of Biotechnology, KIIT Deemed to be University, Bhubaneswar, Odisha, India.
Abstract:
Peroxisomes are ubiquitous organelles with essential roles in lipid and reactive oxygen species (ROS) metabolism. They are involved in modulating the immune responses during microbial infection, thus having major impact on several bacterial and viral infectious diseases including tuberculosis. Intracellular pathogens such as Mycobacterium tuberculosis (M. tb) employ various strategies to suppress the host oxidative stress mechanisms to avoid killing by the host. Peroxisome-mediated ROS balance is crucial for innate immune responses to M. tb. Therefore, peroxisomes represent promising targets for host-directed therapeutics to tuberculosis. Here, we present protocols used in our laboratory for the culture of M. tb and detection of peroxisomal proteins in M. tb infected macrophages.
Insights
Peroxisomes are vital for immune responses against Mycobacterium tuberculosis (M. tb) by regulating reactive oxygen species (ROS). Targeting peroxisomes offers a promising strategy for tuberculosis therapeutics.
Area of Science:
- Cell Biology
- Immunology
- Microbiology
Background:
- Peroxisomes are essential organelles involved in lipid and reactive oxygen species (ROS) metabolism.
- Peroxisomes play a critical role in modulating immune responses to microbial infections, including tuberculosis.
- Intracellular pathogens like Mycobacterium tuberculosis (M. tb) can suppress host oxidative stress mechanisms.
Purpose of the Study:
- To investigate the role of peroxisomes in host immune responses to Mycobacterium tuberculosis.
- To present laboratory protocols for culturing M. tb and detecting peroxisomal proteins in infected macrophages.
Main Methods:
- Culture of Mycobacterium tuberculosis (M. tb) in laboratory settings.
- Detection of peroxisomal proteins in M. tb-infected host macrophages.
Main Results:
- Peroxisome-mediated ROS balance is critical for effective innate immune responses against M. tb.
- The presented protocols enable the study of peroxisomal involvement in M. tb infection.
Conclusions:
- Peroxisomes are crucial players in the host's defense against tuberculosis.
- Peroxisomes represent a potential target for novel host-directed tuberculosis therapies.
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