Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Import into the Peroxisomes01:27

Protein Import into the Peroxisomes

3.6K
Cells contain membrane-bound organelles called peroxisomes that oxidize organic molecules by transferring hydrogen atoms to oxygen, producing hydrogen peroxide. Peroxisomes enzymatically convert the released hydrogen peroxide into water and oxygen.
Peroxisomal Protein Import:
Peroxisomes lack the genetic machinery required to code for their own proteins. Hence, most peroxisomal membrane, lumenal and transmembrane proteins are synthesized in the cytoplasm or ER and transported to the peroxisome...
3.6K
Immunoprecipitation01:20

Immunoprecipitation

5.6K
Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
5.6K
Protein Networks02:26

Protein Networks

4.0K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Visualisation of peroxisomes: a journey through seven decades.

Histochemistry and cell biology·2026
Same author

The neurological pathology of peroxisomal ACBD5 deficiency - lessons from patients and mouse models.

Frontiers in molecular neuroscience·2025
Same author

ROS transfer at peroxisome-mitochondria contact regulates mitochondrial redox.

Science (New York, N.Y.)·2025
Same author

Integrated proteome and lipidome analyses place OCIAD1 at the mitochondria-peroxisome intersection balancing lipid metabolism.

Journal of cell science·2025
Same author

Modelling Peroxisomal Disorders in Zebrafish.

Cells·2025
Same author

Seventy years of peroxisome research: current advances and future perspectives.

Histochemistry and cell biology·2025

Related Experiment Video

Updated: Aug 6, 2025

Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique
07:07

Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique

Published on: July 19, 2019

6.7K

Assessing Peroxisomal Protein Interaction by Immunoprecipitation.

Suzan Kors1, Michael Schrader2

  • 1Faculty of Health and Life Sciences, Biosciences, University of Exeter, Exeter, Devon, UK. s.kors@exeter.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)
|March 23, 2023
PubMed
Summary

This study details immunoprecipitation methods to analyze protein interactions in organelle membrane contacts. These techniques help identify key protein residues regulating interactions, like phosphorylation sites.

Keywords:
ACBD5Co-immunoprecipitationMembrane contact sitesOrganelle interactionsPeroxisomesPhosphorylationProtein binding assayProtein interactionVAPB

More Related Videos

Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
10:05

Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay

Published on: January 16, 2017

12.9K
Author Spotlight: Unraveling the Molecular Mechanisms of Brown and Beige Adipocyte Regulation
07:16

Author Spotlight: Unraveling the Molecular Mechanisms of Brown and Beige Adipocyte Regulation

Published on: January 5, 2024

1.1K

Related Experiment Videos

Last Updated: Aug 6, 2025

Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique
07:07

Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique

Published on: July 19, 2019

6.7K
Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
10:05

Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay

Published on: January 16, 2017

12.9K
Author Spotlight: Unraveling the Molecular Mechanisms of Brown and Beige Adipocyte Regulation
07:16

Author Spotlight: Unraveling the Molecular Mechanisms of Brown and Beige Adipocyte Regulation

Published on: January 5, 2024

1.1K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Organelles interact through protein tethering complexes that bridge membranes.
  • These organelle membrane contacts are dynamic, suggesting dynamic tethering complexes.
  • Studying these interactions is crucial for understanding cellular organization.

Purpose of the Study:

  • To present immunoprecipitation methods for examining protein interaction domains.
  • To identify residues critical for regulating protein interactions, focusing on phosphorylation.
  • To provide a versatile method applicable to various membrane proteins.

Main Methods:

  • Immunoprecipitation using antibody-conjugated beads to purify target proteins and binding partners.
  • Western blot analysis to examine purified protein complexes.
  • Focus on peroxisomal membrane proteins ACBD5 and ACBD4 interacting with ER protein VAPB as a model.

Main Results:

  • Demonstration of immunoprecipitation strategies to assess protein binding alterations.
  • Methodology allows for the identification of specific protein residues involved in interaction regulation.
  • Successful application in studying peroxisome-ER contacts mediated by ACBD5/ACBD4 and VAPB.

Conclusions:

  • Immunoprecipitation is a powerful technique for dissecting protein interactions at organelle contact sites.
  • The presented methods can identify regulatory residues, such as phosphorylation sites, impacting protein binding.
  • This approach is broadly applicable to studying diverse membrane protein interactions in cell biology.