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Related Experiment Videos

Structure and function of the glucocorticoid receptor.

J Carlstedt-Duke1, J A Gustafsson

  • 1Department of Medical Nutrition, Karolinska Institute, Huddinge University Hospital, Sweden.

Journal of Steroid Biochemistry
|January 1, 1987
PubMed
Summary
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The glucocorticoid receptor protein binds DNA enhancers equally and is regulated by glucocorticoids. Its DNA-binding and steroid-binding domains are key to its function.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • The glucocorticoid receptor (GR) is a crucial transcriptional regulator.
  • GR activity is modulated by glucocorticoid binding.
  • Understanding GR's interaction with DNA is vital for gene regulation studies.

Purpose of the Study:

  • To investigate the stoichiometric relationship between the glucocorticoid receptor protein and enhancer sequences.
  • To define the functional domains of the glucocorticoid receptor at the protein and sequence level.
  • To elucidate the regulatory mechanisms of glucocorticoid receptor activity.

Main Methods:

  • Stoichiometric analysis of protein-DNA interactions.
  • Functional domain mapping of the glucocorticoid receptor.

Related Experiment Videos

  • Sequence-level analysis of functional domains.

    • Main Results:

    • An equimolar relationship was observed between the glucocorticoid receptor protein and specific enhancer sequences.
    • The DNA-binding and steroid-binding functional domains are located in the C-terminal half of the receptor.
    • The N-terminal half modulates DNA interaction but not steroid binding.
    • Functional domains were precisely defined at the sequence level.

    Conclusions:

    • Glucocorticoid receptor (GR) interaction with DNA enhancers is stoichiometric.
    • GR functional domains are separable and located in the C-terminal region.
    • The N-terminal region plays a modulatory role in DNA binding, while the C-terminal region houses both DNA and steroid binding sites.