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Related Experiment Videos

A reservoir model for repeated CSF access in the rabbit.

M A Samphilipo1, S J Hassenbusch, L B Grochow

  • 1Department of Radiology, Johns Hopkins Medical Institutions, Baltimore, MD 21205.

Journal of Neuroscience Methods
|November 1, 1987
PubMed
Summary

Researchers developed a simple, reliable, and inexpensive method for repeated sterile access to the rabbit brain ventricular system, enabling drug delivery and cerebrospinal fluid withdrawal.

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Area of Science:

  • Neurosurgery
  • Animal Models
  • Drug Delivery Systems

Background:

  • Accessing the rabbit brain ventricular system for repeated drug administration or cerebrospinal fluid (CSF) withdrawal is challenging.
  • Existing methods may be complex, unreliable, or costly, limiting their widespread use in research.

Purpose of the Study:

  • To develop and evaluate a simple, reliable, and inexpensive subcutaneously implanted reservoir-catheter system for the rabbit brain.
  • To enable repeated sterile access to the rabbit's ventricular system for intrathecal drug administration and CSF withdrawal.

Main Methods:

  • A reservoir-catheter system was designed for subcutaneous implantation under the scalp in rabbits.
  • The catheter was inserted into either the cisterna magna or the lateral ventricle.

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  • System patency was assessed using X-rays and radiopaque contrast medium.

    • Main Results:

    • The system functioned successfully in 38 out of 41 rabbits (92.7% success rate).
    • The system remained functional for up to 12 weeks post-implantation.
    • Both cisternal magna and lateral ventricle catheter placements demonstrated successful outcomes.

    Conclusions:

    • The developed reservoir-catheter system offers a simple, reliable, and cost-effective solution for repeated access to the rabbit brain ventricular system.
    • This method facilitates intrathecal drug delivery and CSF withdrawal in preclinical research.
    • The system's high success rate and long-term patency support its utility in neuroscience studies.