Related Experiment Videos
A perfusion chamber for physiological studies with acutely dissociated neurons
1Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Baltimore 21201.
Journal of Neuroscience Methods
|November 1, 1987
Summary
A novel recording chamber immobilizes neurons for stable electrophysiological recordings. This design facilitates precise voltage-clamp and patch-clamp studies with rapid perfusion.
Area of Science:
- Neuroscience
- Biophysics
- Electrophysiology
Background:
- Electrophysiological recordings require stable neuronal immobilization.
- Rapid perfusion is crucial for studying dynamic cellular responses.
Purpose of the Study:
- To develop an easily fabricated recording chamber for enhanced neuronal electrophysiology.
- To enable stable single-electrode voltage-clamp and patch-clamp recordings.
Main Methods:
- Fabrication of a novel recording chamber utilizing an ultra-fine mesh grid.
- Positioning the mesh grid above a moving perfusate stream.
- Achieving shallow immersion (<20 microns) of acutely dissociated neurons.
Main Results:
- The chamber effectively immobilizes neurons.
- Shallow immersion and stable platform enable high-fidelity recordings.
- The design supports rapid perfusion rates for dynamic experiments.
Conclusions:
- This recording chamber offers a practical solution for stable neuronal electrophysiology.
- It enhances the capability for detailed study of neuronal ion channel function.
- The design is suitable for both voltage-clamp and patch-clamp techniques.