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Related Experiment Videos

Polyamine changes during senescence and tumorogenesis in plants.

B I Srivastava1

  • 1Department of Laboratory Medicine, Roswell Park Memorial Institute, Buffalo, NY 14263.

Mechanisms of Ageing and Development
|September 14, 1987
PubMed
Summary

Polyamines like putrescine, spermidine, and spermine are present in barley and tobacco tissues. Their levels do not consistently change during plant senescence, suggesting they are not natural anti-senescent compounds.

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Area of Science:

  • Plant Physiology
  • Biochemistry
  • Molecular Biology

Background:

  • Polyamines (putrescine, spermidine, spermine) are crucial for plant growth and development.
  • Their role in plant senescence, particularly in barley (Hordeum vulgare L.) and tobacco (Nicotiana tabacum), requires further elucidation.
  • Previous research suggests potential roles in enzyme activity modulation and senescence retardation.

Purpose of the Study:

  • To investigate the levels of polyamines in barley and tobacco tissues during different developmental stages, including senescence.
  • To determine the effect of polyamines on senescence and associated enzyme activities (RNase, DNase, phosphodiesterase) in barley.
  • To assess the potential of polyamines as natural anti-senescent compounds in plants.

Main Methods:

Related Experiment Videos

  • Quantification of polyamines (putrescine, spermidine, spermine) in barley seedling leaves under light/dark conditions and during senescence.
  • Treatment of excised barley leaves with polyamines and kinetin to assess effects on senescence and enzyme activities.
  • Analysis of polyamine levels in normal, crown gall tumor, and TMV-infected tobacco tissue cultures.

    • Main Results:

    • Polyamines were detected in barley and tobacco tissues, with higher levels in actively growing or cultured tissues compared to non-proliferating ones.
    • No significant, consistent changes in polyamine levels were observed during barley leaf senescence, except for a possible decline in spermidine.
    • Polyamines inhibited RNase and DNase activities but stimulated phosphodiesterase activity in vitro; however, these effects did not correlate with anti-senescence activity.

    Conclusions:

    • The study does not support the hypothesis that polyamines are natural anti-senescent compounds in plants.
    • The observed in vitro enzyme inhibition by polyamines is unlikely to be the mechanism behind any potential anti-senescence effects.
    • High polyamine levels are associated with actively growing plant tissues, but their role in senescence remains inconclusive.