Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cholesterol: Significance and Regulation01:29

Cholesterol: Significance and Regulation

616
Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...
616
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

2.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.4K
Transducer Mechanism: Nuclear Receptors01:31

Transducer Mechanism: Nuclear Receptors

1.4K
Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
1.4K
Oxidation of Phenols to Quinones01:17

Oxidation of Phenols to Quinones

3.3K
In the presence of oxidizing agents, phenols are oxidized to quinones. Quinones can be easily reduced back to phenols using mild reducing agents. The electron-donating hydroxyl group enhances the reactivity of the aromatic ring, enabling oxidation of the ring even in the absence of an α hydrogen.
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox...
3.3K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

7.7K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
7.7K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

2.1K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diagnostic and decision-making disparities in precision oncology in thoracic malignancies with interstitial pneumonia: A real-world cohort study.

Respiratory investigation·2026
Same author

Navigational Bronchoscopy Training With Spatial Computing: A Simulation-Based Feasibility Report Using Apple Vision Pro.

Surgical innovation·2026
Same author

Comparison of biomechanical assessment between upright and supine inspiratory-expiratory area-detector CT in progressive pulmonary fibrosis.

European radiology experimental·2026
Same author

Initial Experience with Three-Dimensional Holographic Virtual Bronchoscopy Using Mixed Reality for Peripheral Pulmonary Lesions: A Retrospective Feasibility Study.

Respiration; international review of thoracic diseases·2026
Same author

Epigenetic Scarring and a Vulnerable Mucus-Barrier Axis in Ex-Smokers With COPD.

Respirology (Carlton, Vic.)·2026
Same author

Favorable clinical impact of histological subtype with non-small cell carcinoma-not otherwise specified in patients with non-small cell lung cancer receiving immune checkpoint inhibitors.

Respiratory investigation·2026

Related Experiment Video

Updated: Aug 5, 2025

Cholesterol Efflux Assay
07:54

Cholesterol Efflux Assay

Published on: March 6, 2012

29.9K

Decrease in cholesterol in the cell membrane is essential for Nrf2 activation by quercetin.

Miyoko Matsushima1, Haruka Nose1, Hikaru Tsuzuki1

  • 1Division of Host Defense Sciences, Omics Health Sciences, Department of Integrated Health Sciences, Nagoya University Graduate School of Medicine, Tokai National Higher Education and Research System, Nagoya, Japan.

The Journal of Nutritional Biochemistry
|March 23, 2023
PubMed
Summary

Quercetin induces protective heme oxygenase-1 (HO-1) by altering cell membrane lipid rafts. This involves cholesterol reduction, promoting the nuclear translocation of the caveolin-1 (Cav-1)-Nrf2 complex, and enhancing antioxidant capacity.

Keywords:
CaveolinCholesterolHeme oxygenase-1Lipid raftsNrf2Quercetin

More Related Videos

Macrophage Cholesterol Depletion and Its Effect on the Phagocytosis of Cryptococcus neoformans
11:07

Macrophage Cholesterol Depletion and Its Effect on the Phagocytosis of Cryptococcus neoformans

Published on: December 19, 2014

12.8K
High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay
08:18

High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay

Published on: January 7, 2019

9.1K

Related Experiment Videos

Last Updated: Aug 5, 2025

Cholesterol Efflux Assay
07:54

Cholesterol Efflux Assay

Published on: March 6, 2012

29.9K
Macrophage Cholesterol Depletion and Its Effect on the Phagocytosis of Cryptococcus neoformans
11:07

Macrophage Cholesterol Depletion and Its Effect on the Phagocytosis of Cryptococcus neoformans

Published on: December 19, 2014

12.8K
High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay
08:18

High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay

Published on: January 7, 2019

9.1K

Area of Science:

  • Cell Biology
  • Biochemistry
  • Molecular Pharmacology

Background:

  • Quercetin, a flavonoid, exhibits cytoprotective effects, including anti-allergic, anti-oxidative, and anti-fibrotic activities.
  • These activities are mediated through the induction of heme oxygenase-1 (HO-1).
  • The precise mechanisms by which quercetin induces HO-1 remain incompletely understood, particularly concerning its interaction with the cell membrane.

Purpose of the Study:

  • To investigate the role of cell membrane structural changes in quercetin-induced HO-1 expression.
  • To elucidate the signaling pathway involving lipid rafts, caveolin-1 (Cav-1), and nuclear factor E2-related factor 2 (Nrf2) in response to quercetin.

Main Methods:

  • Analysis of lipid raft composition and cholesterol content in cell membranes after quercetin treatment.
  • Assessment of Cav-1 and Nrf2 expression and localization using cell fractionation and nuclear translocation assays.
  • Measurement of HO-1 induction in response to quercetin and associated membrane changes.

Main Results:

  • Quercetin treatment decreased cholesterol levels in raft fractions, promoting HO-1 induction.
  • Alterations in lipid raft composition and differential expression of Cav-1 in raft and non-raft fractions were observed.
  • Quercetin induced the translocation of Nrf2 and Cav-1 from the cell membrane to the nucleus.

Conclusions:

  • Quercetin's HO-1-dependent cytoprotective effects are mediated by structural modifications of lipid rafts.
  • Decreased cell membrane cholesterol content by quercetin facilitates the translocation of the Cav-1-Nrf2 complex to the nucleus.
  • This translocation ultimately leads to the induction of HO-1 and subsequent cytoprotection.