Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

5.6K
The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
5.6K
Nucleosome Remodeling02:54

Nucleosome Remodeling

9.3K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
9.3K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

8.3K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
8.3K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

6.3K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.3K
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

1.7K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.7K
Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

2.8K
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antibody-oligonucleotide conjugates: an emerging modality for precision RNA therapeutics.

Antibody therapeutics·2026
Same author

Transcription elongation can be sufficient, but is not necessary, to advance replication timing.

EMBO reports·2026
Same author

Netrin-1 Promotes Pancreatic Tumorigenesis and Innervation through NEO1.

Cancer research·2025
Same author

An integrated view of the structure and function of the human 4D nucleome.

Nature·2025
Same author

mRNA Vaccines: Current Applications and Future Directions.

MedComm·2025
Same author

Chlorogenic acid ameliorates chronic stress-induced depression-like behaviors in rats by inhibiting oxidative stress and neuroinflammation via the PI3K/Akt/Nrf2 pathway.

Cellular signalling·2025
Same journal

Identification of chemical features for improved outer membrane permeation in mycobacteria using machine learning.

Nature microbiology·2026
Same journal

Author Correction: Gut commensal Christensenella minuta modulates host metabolism via acylated secondary bile acids.

Nature microbiology·2026
Same journal

Mobile genetic elements shape microbial diversity and functions in thawing permafrost soils.

Nature microbiology·2026
Same journal

Epistatic interactions inform rational design of synthetic microbial communities for bioremediation.

Nature microbiology·2026
Same journal

END nucleases are antiphage defence systems targeting multiple phages with modified genomes.

Nature microbiology·2026
Same journal

Complex multicellularity is linked with expanded specialized metabolite production in microorganisms.

Nature microbiology·2026
See all related articles

Related Experiment Video

Updated: Aug 5, 2025

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

6.5K

SARS-CoV-2 restructures host chromatin architecture.

Ruoyu Wang1,2, Joo-Hyung Lee1, Jieun Kim3,4

  • 1Department of Biochemistry and Molecular Biology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX, USA.

Nature Microbiology
|March 24, 2023
PubMed
Summary
This summary is machine-generated.

Severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) infection restructures host cell chromatin, altering gene expression. This virus disrupts the 3D genome and epigenome, unlike common cold viruses.

More Related Videos

CRISPR-Mediated Reorganization of Chromatin Loop Structure
09:20

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Published on: September 14, 2018

12.6K
Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
05:23

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Published on: December 23, 2020

6.1K

Related Experiment Videos

Last Updated: Aug 5, 2025

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

6.5K
CRISPR-Mediated Reorganization of Chromatin Loop Structure
09:20

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Published on: September 14, 2018

12.6K
Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
05:23

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Published on: December 23, 2020

6.1K

Area of Science:

  • Genomics
  • Epigenetics
  • Virology

Background:

  • Viral infections can alter host cell gene expression by modifying chromatin structure.
  • The impact of SARS-CoV-2 on the host cell's 3D genome and epigenome remains largely uncharacterized.

Purpose of the Study:

  • To investigate the effects of SARS-CoV-2 infection on the 3D genome and epigenome of human cells.
  • To compare SARS-CoV-2-induced changes with those caused by other coronaviruses.

Main Methods:

  • Characterization of the 3D genome and epigenome in human cells post-SARS-CoV-2 infection.
  • Analysis of chromatin compartment changes, intra-TAD contacts, and histone modifications (H3K27ac, H3K4me3).
  • Assessment of cohesin complex localization and its role in loop extrusion.

Main Results:

  • SARS-CoV-2 infection caused widespread host chromatin restructuring, including compartment A weakening, A-B mixing, and reduced intra-TAD contacts.
  • Decreased levels of H3K27ac euchromatin modification were observed, alongside depletion of the cohesin complex from intra-TAD regions.
  • These epigenetic alterations correlated with transcriptional suppression of interferon genes and increased H3K4me3 at promoters of pro-inflammatory genes.

Conclusions:

  • SARS-CoV-2 acutely rewires the host cell's 3D genome and epigenome.
  • The virus disrupts cohesin-mediated loop extrusion, contributing to altered gene expression patterns.
  • Findings provide a basis for studying the long-term epigenomic consequences of SARS-CoV-2 infection.