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A versatile strategy for convenient circular bivalent functional nucleic acids construction.

Xiao-Jing Zhang1, Zhuo Zhao1, Xia Wang1

  • 1Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha 410082, China.

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Summary

Researchers developed a new chemical strategy to modify functional nucleic acids (FNAs). This method efficiently creates circular bivalent aptamers (CBApts) with improved stability and targeting for biomedical applications.

Keywords:
chemical ligationcircular bivalent aptamersclick chemistry

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Chemical Biology

Background:

  • Functional nucleic acids (FNAs) like aptamers are crucial in life sciences.
  • Chemical modification of FNAs can enhance their biomedical properties and applications.
  • Current methods for FNA tailoring have limitations in scope and efficiency.

Purpose of the Study:

  • To develop a general chemical tailoring strategy for DNA sequences.
  • To create a method for efficient synthesis of designed circular bivalent aptamers (CBApts).
  • To evaluate the enhanced properties of CBApts for biomedical applications.

Main Methods:

  • A novel divergent ligation strategy for DNA sequence modification.
  • Application of the strategy to synthesize various aptamers and cyclized DNA sequences.
  • Characterization of circular bivalent aptamers (CBApts) for stability and binding.

Main Results:

  • The chemical tailoring strategy enabled efficient synthesis of DNA sequences with high yields.
  • Circular bivalent aptamers (CBApts) were successfully designed and produced.
  • CBApts demonstrated significantly enhanced nuclease resistance.
  • CBApts showed improved binding affinity, targeting, and tumor tissue enrichment.

Conclusions:

  • A versatile chemical tailoring strategy for DNA sequences has been established.
  • The developed method allows for the efficient creation of circular bivalent aptamers (CBApts).
  • CBApts possess superior biomedical properties, including enhanced stability and targeting, suggesting significant potential for clinical translation.