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Programmable Tissue Folding Patterns in Structured Hydrogels.

Avinava Roy1, Zenghao Zhang1, Madeline K Eiken2

  • 1Department of Materials Science & Engineering, University of Michigan, North Campus Research Complex, 2800 Plymouth Rd, Ann Arbor, MI, 48109, USA.

Advanced Materials (Deerfield Beach, Fla.)
|March 24, 2023
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Summary

Researchers developed a novel bilayer hydrogel system to mimic dynamic tissue folding in vitro. This platform enables the study of folded tissue patterns

Keywords:
airwayshydrogelsmagnetoactive hydrogelsmicroactuatorstissue folding

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Cell Biology

Background:

  • Tissue folding is crucial for organ function, particularly in epithelial tissues like the respiratory airways.
  • Existing cell culture methods lack the ability to replicate dynamic tissue folding in vitro, hindering research into cellular responses to folded tissue patterns.

Purpose of the Study:

  • To develop a dynamic in vitro cell culture platform that can recreate tissue folding.
  • To investigate the influence of engineered folded tissue patterns on cellular function.

Main Methods:

  • A bilayer hydrogel system was created using alginate/polyacrylamide double-network (DN) and hyaluronic acid (HA) hydrogels.
  • Human fibroblasts were encapsulated in patterned HA hydrogels, leading to the formation of folded pseudostratified human bronchial epithelial cell monolayers.
  • Magnetic microparticles were incorporated into DN hydrogels, allowing for magnetically controlled reversible folding of cell-laden hydrogel systems.

Main Results:

  • The bilayer hydrogel system successfully generated static and dynamic folding patterns.
  • Human bronchial epithelial cells formed folded pseudostratified monolayers on the patterned hydrogels.
  • Programmable, on-demand folding of cell-laden hydrogels was achieved using an external magnetic field.

Conclusions:

  • The developed hydrogel system provides a dynamic platform for mimicking tissue folding in vitro.
  • This technology is adaptable for studying various cell types and organ systems, advancing research in tissue engineering and regenerative medicine.