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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Peptidomics: LC-MS operational parameters do matter.

Amélie Descamps1, Kevin Van der Borght2, Anton De Spiegeleer3

  • 1Drug Quality and Registration (DruQuaR) group, Department of Pharmaceutical Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium; Translational Research in Immunosenescence, Gerontology and Geriatrics (TRIGG) group, Ghent University Hospital, Corneel Heymanslaan 10, 9000 Ghent, Belgium.

Journal of Pharmaceutical and Biomedical Analysis
|March 24, 2023
PubMed
Summary
This summary is machine-generated.

Sensitive detection of quorum sensing peptides in biofluids is crucial for disease research. This study recommends novel analytical approaches to improve peptidomic measurements, enhancing lab-to-human translation for various low-concentration peptides.

Keywords:
AQbDBio-analysisLC-MSMicrobiomePeptidomicsQuorum Sensing Peptides

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology

Background:

  • Sensitive detection of low-level peptides in biofluids is essential for translating peptidomic research findings to clinical applications.
  • Quorum sensing peptides are increasingly implicated in human diseases, necessitating reliable analytical methods for their study.

Purpose of the Study:

  • To investigate and recommend novel approaches for critical, often overlooked, steps in liquid chromatography-mass spectrometry (LC-MS) based peptidomic analysis.
  • To enhance the accuracy and reliability of measuring peptides, particularly quorum sensing peptides, in biological samples.

Main Methods:

  • Application of a Quality by Design (QbD) approach to peptidomic analysis.
  • Investigation of sample stabilization techniques.
  • Development and testing of peptide anti-adsorption tools.
  • Optimization of injection solvent, gradient system, and duty cycle parameters for LC-MS.

Main Results:

  • Novel, QbD-approached solutions were developed for key challenges in peptidomic bio-analytics.
  • Recommended strategies demonstrated potential to improve the measurement of low-concentration peptides, using quorum sensing peptides as models.
  • The study identified optimal parameters for sample handling, LC separation, and MS detection.

Conclusions:

  • The recommended analytical strategies significantly improve the bio-analytics of quorum sensing peptides.
  • These advancements are valuable for peptidomic research, particularly for detecting low-concentration analytes.
  • The findings facilitate better lab-to-human translation of peptidomic discoveries for various disease-related peptides.