Related Experiment Video
Updated: Jul 30, 2026

Physiology Lab Demonstration: Glomerular Filtration Rate in a Rat
Published on: July 26, 2015
Plasma hippurate in renal failure: high-performance liquid chromatography method and clinical application
P Igarashi1, P Gulyassy, L Stanfel
1Department of Internal Medicine, University of California, Davis.
Researchers developed a precise HPLC method to measure hippurate in renal failure patients. Elevated hippurate levels correlate with kidney dysfunction and may cause albumin binding issues.
Area of Science:
- Clinical Chemistry
- Analytical Chemistry
- Nephrology
Background:
- Hippurate accumulation in renal failure contributes to impaired drug-protein binding.
- Azotemic patients may experience other health issues due to elevated hippurate.
Purpose of the Study:
- To develop and validate a high-performance liquid chromatography (HPLC) method for quantifying plasma hippurate.
- To investigate the relationship between hippurate levels and renal function markers.
Main Methods:
- High-performance liquid chromatography (HPLC) was employed for hippurate assay.
- Plasma samples from 25 patients with acute and chronic renal failure were analyzed.
Main Results:
- The HPLC method demonstrated precision, accuracy, and reproducibility.
- Plasma hippurate concentrations ranged from 0.11 to 16.2 mg/dl.
- Hippurate levels showed a moderate correlation with serum creatinine, urea, and anion gap.
Conclusions:
- The developed HPLC method is suitable for assessing hippurate in renal failure patients.
- Hippurate accumulation is linked to declining GFR and tubular function.
- This assay can aid further research into abnormal albumin binding and azotemic complications.
More Related Videos
13:35A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites
Published on: March 1, 2018
14:39Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults
Published on: April 22, 2022
Related Concept Videos
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Instrumentation
Renal Clearance
Renal clearance refers to the volume of plasma cleared of a specific substance, such as creatinine, per unit of time. To measure clearance, urine samples are collected over a 24-hour period during each bladder voiding, followed by a single blood sample at the...
Renal Drug Excretion: Tubular Secretion
Extracorporeal Removal of Drugs: Peritoneal Dialysis and Hemodialysis
Extracorporeal Removal of Drugs: Hemoperfusion and Hemofiltration