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Updated: Aug 5, 2025
Development and Functional Characterization of Murine Tolerogenic Dendritic Cells
Published on: May 18, 2018
Effects of simulated digestion on the structural characteristics and dendritic cell activation of longan
Shengwei Wang1, Juncheng Chen2, Chao Feng3
1Key Laboratory of Food Nutrition and Functional Food of Hainan Province, College of Food Science and Engineering, Hainan University, Haikou 570228, China; School of Biotechnology and Health Sciences, Wuyi University, Jiangmen 529020, China.
Abstract:
An active polysaccharide (LP) from longan was purified and characterized. LP consisted of galactose and glucose in a molar ratio of 1.5: 98.5, with a molecular weight of 4.67 × 107 g/mol. The main backbone of LP was T-α-D-Glcp-[(1 → 6)-α-D-Glcp-(1 → 6)-α-D-Glcp]n. After simulated gastrointestinal digestion, the molecular weight distribution, monosaccharide composition, and major glycosidic bonds of LP were not significantly changed. LP and digested LP (DLP) reduced phagocytosis and promoted IL-10 and IL-12 secretion of dendritic cells. In addition, the effects of LP and DLP on activating dendritic cells showed no significant difference. This study helps to illuminate the potential mode of immunomodulatory action of longan polysaccharides in vivo.
