Water-soluble PEG segmented mannose-based macromolecules: Synthesis, characterization and their biocompatibility
N Naga Malleswara Rao1, Krushna K Palodkar1, T Sandeep Kumar2
1Polymers and Functional Materials and Fluoro-Agrochemicals Department, CSIR-Indian Institute of Chemical Technology, Uppal Road, Hyderabad 500007, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, Uttar Pradesh, India.
International Journal of Biological Macromolecules
|March 24, 2023
Summary
Novel mannose-based polymers were synthesized and deacetylated to create water-soluble, biocompatible materials. These glycopolymers show promise for enhancing cell proliferation and adhesion, particularly the ABA type copolymer for osteointegration.
Area of Science:
- Polymer Chemistry
- Biomaterials Science
- Cell Biology
Background:
- Development of biocompatible and water-soluble macromolecules is crucial for biomedical applications.
- Glycopolymers, incorporating carbohydrate moieties, offer unique biological recognition properties.
- Mannose-based polymers are of interest for their potential to interact with specific cell surface receptors.
Purpose of the Study:
- To synthesize novel mannose-based methacrylate and PEG block copolymers (AB and ABA types).
- To evaluate the physicochemical properties and biocompatibility of the resulting deacetylated glycopolymers.
- To assess the potential of these glycopolymers in promoting cell proliferation, adhesion, and osteointegration.
Main Methods:
- Synthesis of AB and ABA type block copolymers via atom transfer radical polymerization (ATRP).
- Deacetylation to yield water-soluble glycopolymer macromolecules.
- Characterization using GPC, proton NMR, TGA, and DSC.
- Biocompatibility assessment using Human Bone Derived Cells (HBDC) and MTS assay.
Main Results:
- Synthesized acetylated and deacetylated macromolecules with controlled molecular weights.
- Deacetylated glycopolymers exhibited water solubility and thermal stability.
- ABA type diblock copolymers demonstrated enhanced HBDC mitochondrial activity and proliferation, indicating good biocompatibility and potential for osteointegration.
Conclusions:
- Novel mannose-based glycopolymer architectures (AB and ABA) were successfully synthesized and characterized.
- The water-soluble, biocompatible glycopolymers show potential for improving cell proliferation and adhesion.
- The ABA type copolymer architecture is particularly promising for enhancing osteointegration efficiency.
