Co-fermenting lactic acid and glucose towards caproic acid production

The functional role of lactate (H_Lac), as a co-substrate along with glucose (Glu) as well as an electron donor for the synthesis of caproic acid (H_Ca), a medium chain fatty acid (MCFAs) was studied. A varied H_Lac and Glu ratios were thus investigated in fed-batch anaerobic reactors (R1-R5) operated at pH 6 with a heat-treated anaerobic consortium. R1 and R5 were noted as controls and operated with sole Glu and H_Lac, respectively. Strategically, ethanol (H_Eth) was additionally supplemented as co-electron donor after the production of short chain carboxylic acids (SCCAs) for chain elongation in all the reactors. The reactor operated with H_Lac and Glu in a ratio of 0.25:0.75 (1.25 g/L (H_Lac) and 3.75 g/L (Glu)) showed the highest H_Ca production of 1.86 g/L. R5 operated with solely H_Lac yielded propionic acid (H_Pr) as the major product which further led to the higher valeric acid (H_Va) production of 1.1 g/L within the reactor. Butyric acid (H_Bu) was observed in R1, which used Glu as carbon source alone indicating the importance of H_Lac as electron co-donor. Clostridium observed as the most dominant genera in shotgun metagenome sequencing in R2 and R3, the reactors that produced the highest H_Ca in comparison to other studied reactors. The study thus provided insight into the importance of substrate and electron donor and their supplementation strategies during the production of MCFAs.